Acute Toxicity Of Bisphenol A To Tadpole (Rana Chensinensis) And Its Estrogenic Effect In The Sexual Differentiantion Of Gonad

with the rapid development of industry, Phenol aggravates to environmental pollution day by day. Bisphenol A (BPA) is one kind of degraded production of industrial chemicals. In the high concentration, it has acute toxicity to cause the tadpole (Rana chensinensis) directly dead. However it can disrupt animal's endocrine function in low concentration, causing unusual growth, abnormal development and dysfunction of reproduction. Thus it influences the normal procreation of the population. In our article, the acute toxicity, anti-metamorphic effects of BPA to tadpole and estrogenic effects on sexual differentiation of gonad were assessed, it aimed to investigate the mechanism of estrogenic effects of BPA and show the foundational theory for environmental protection.The tadpoles( Rana chensinensis) were divided into BPA dosed adults and wild group.They were exposed to different concentrations of BPA during larval development. After metamorphosis, survival rate, length, weight, the time of complete to metamorphosis and sex ratio were counted in order to assess its toxicity effects on growth, development, and its estrogenic effects on sexual differentiation of gonad. The expression of ER, AR, Aromatase in gonad and the expression of ER in liver were detected by immunocytochemistry. The main results showed as following:1. Its 24-hour, 48-hour, 72-hour LD50 for tadpoles were 3.17×10-5M, 2.993× 10-5M and 2.854×10-5M, its SD was 0.8009×10-5M. It showed that the death rate of tadpole, with the increasing of concentration, had increased above safe concentration of BPA.2. Under the safe concentration, BPA had no effect on survival rate of larval after metamorphosis in 10-5, 10-6,10-7M. In BPA dosed adults group, BPA caused larval's length to reduce in 10-5M. In the wild group, it did not influence the length of larval. It proved that the adult frogs were infected by BPA, the length of its generation had reduced in a certain range of BPA.In 10-5M, BPA obviously reduced the weight of larval in both BPA dosed adults group and wild group. However, in other concentrations, BPA had no obvious influence on the weight of larval in either BPA dosed adults group or wild group. It proved that BPA caused larval's weight to reduce only in a certain range of concentrations.3. In 10-5~10-6M, BPA lengthened the time of complete to metamorphosis. With the increasing of BPA concentration, the obvious postponement of complete metamorphosis had been noticed. BPA had no remarkable difference in anti-metamorphic effects from BPA dosed adults and wild group. It showed that BPA...