Studies On The Application Of Solid Phase Microextraction In The Analysis Of Organochlorine Pesticides

Marine organisms ( Mussels and other bivalve mollusks ) have the ability toaccumulate trace level of certain pollutants, such as organochlorine pesticides, heavy metalsetc, from their environment. After human being took the Mussels as food, pesticides weretransferred into human bodies and did great harm to their health. The traditional methoddesigned to cleaning up and concentrating the organochlorine pesticides in seawater islaborious and time consuming. In this paper, a simple and quick method has been establishedbased on the combination among SPME, gas chromatography and electron capturing detector.Solid-phase microextraction (SPME) is a novel solvent-free analytical technique, ableto integrate extraction, concentration and sample introduction in a single step. Thus, it hasbeen proved to be significantly more rapid, simple and easier. Especially PDMS (100μm) ismore suitable for adsorbing and enriching the OCPs in the seawater after its comparison withthe other two fibers, PDMS-DVB(65μm)and PA(85μm). In this paper, experimentalcondition of SPME were also thoroughly optimized, including extraction time, extractiontemperature, and desorption time, etc.Under the method established, the conditions of chromatographicseparation and the detection were also discussed, including the choice of thechromatography column through the way of thermodynamic, type of detector, etc.The optimized method has been employed to detect the presence of organochlorinepesticides in the offshore seawater of culturing area. it has been proved to besimple, fast and accurate.