Fluoremetric Methods For The Deteriantion Of Quinolones And Studies On The Interaction Of Quinolone With Metal Ions And Bovine Serum Albumin

Pharmaceutical analysis is most popular subject both in life science and analytical chemistry, including the quality control of pharmaceutical preparations as soon as studies on the biological procedure of drugs in the organisms. Fluoremetric methods have a promising future in the pharmaceutical analysis area, with the merit of high sensitivity, high selectivity and more simple then many other methods. This thesis carried out following two part of work focusing on the quinolones.1. The fluorescence characters of quinolones were studied in detail and the variables of experiment were optimized. It had been found that the sensitivity of methods were greatly increased after the use of micellar enhanced fluoremetry and metal ion enhanced fluoremetry. Based on this fluoremetric methods for the determination of quinolones in pharmaceutical preparations were proposed. Most biological fluids are fluorescent and provide high background fluorescence which overlap the spectra of quinolone in most circumstance. However these spectra overlaps were successfully resolved by applying synchronous fluoremetry and zero-crossing synchronous derivative fluoremetry. Based on this two fluoremetric methods for the determination of the quinolones in human urine without tedious pre-separation were proposed. Proposed methods are simple, accurate and sensitive, and were applied satisfactorily to the determination of quinolones in tablets, capsules, injections and human urine samples. The recoveries were withinthe range of 95%~100%.2. The interactions between new quinolone Gatifloxacin and eight metal ions were studied spectraphotometrically and fluoremetrically. Experimental results indicate all eight metal ions form complexes with gatifloxacin in human body pH range. Conformation and conditional formation constant were determined using logarithm plot method, and the interaction sites were discussed. The interaction between gatifloxacin and bovine serum albumin (BSA) were studied also. Inner filter effect was corrected by partial least square procedure. Quenching constant was obtained by plotting stern-volmer plot and the quenching mechanism was discussed. It was proposed that the quenching sphere action might be the real cause of fluorescence quenching other then ground state complex formation. The distance between Gatifloxacin and BSA was determined according to theory of energy transfer. This part of work is usefull for the knowledge of existing forms, distribution, absorption and mechanism of biological effects of this drug, and provide guide to the work of putting forward a appropriate dosage regimen and developing new quinolones with more antibioactivity.