Application Of System Dynamics On The Simulation Of Biosynthesis Process Of L-phenylalanine

If we establish a dynamic simulation model of biochemical reaction process, we can preview or playback a biochemical reaction process and change the kinetic parameters and control schemes of the reaction in real time. After finding the optimal scheme we come back to experiment and validate it. It saves time and materials of experiment. Consequently the simulation model of biochemical reaction process has important values not only in theoretical research but also in industrial production. L-phenylalanine is one of the eight amino acids that are needed by human body and has the extensive application in the medicine and food field. Biosynthesis is commonly used to produce L-phenylalanine industrially. The complexity of the structure and behavior of biology system results in the complexity of internal mechanism of biochemical reaction process and the coupling of reaction parameters. On the basis of systems biology, we used system dynamics software ITHINK as the modeling tool in this article and solved the difficult problem of non-linearity and time lag. In this article we established simulation models of two kinds of methods of biosynthesis of L-phenylalanine: zymotechnics and enzyme respectively and then validated and modified the models with experimental data. We designed many different operation and regulation conditions to simulate their effect to the yield of L-phenylalanine. The simulation results corresponded with the experimental data very well. This simulation model can offer system dynamic analysis for biosynthesis of L-phenylalanine and powerful help for evaluating the merits and demerits of all kinds of regulation and control schemes. It can also be used to find new optimization means.