Studies On Preparation And Hydrolysis Activities Of The Complex Particles Made From Agarose And Ce(â…£)

Rare earth elements (REE) can hydrolyze RNA and DNA. Especially, the hydrolysis activity of Ce(â…£) is more than that of other REE, and only phosphoester bonds of DNA or RNA can be hydrolyzed by Ce(â…£). Furthermore, some oxidation byproducts can't be produced during the hydrolysis reaction. Whereas, a great deal of steady gelatinous Ce(â…£)-OH deposit will be formed at physiological pH so as to reduce the solvency of Ce(â…£). Therefore, one suitable ligand which can react with Ce(â…£) must be found in order to broaden its purpose.Agarose, which is a kind of catenarian amylose, is consisted of D-galactose and 3,6-dehydration-L-galactose. And agarose particles are a sort of ideal chromatography medium. Therefore, particles of agarose- Ce(â…£) complexes (PACC) are obtained by twice emulsification by making use of agarose as ligand and tween-80, span-85 and cyclohexane as emulsion. PACC are primarily described by micrography, UV spectrum, IR spectrum and fluorescence spectrum. The using environment of the PACC such as pH, temperature,acetone, ethlylacetate, centrifugal effect and ultrasonic effect are discussed in this paper.At the same time, hydrolysis activities of PACC for phosphoester and peptide bonds are mainly studied. The results show that PNPP2Na, which is regarded as the model compound of phosphoester bonds, can be hydrolyzed by PACC. Furthermore, the hydrolysis activities of PACC for organophosphorus pesticides which have phosphoester bonds are also discussed. The degraded rates of methamidophos,omethoate and chlorpyrifos are 32.39%, 27.12% and 46.62% respectively. And PACC candegrade organophosphorus pesticide residues consisting in the tea extract and sprout juice. The degraded rate of chlorpyrifos in the tea extract is 59.76%. And the degraded rates of chlorpyrifos and methamidophos in sprout juice are 38.28% and 35.45% respectively.The hydrolysis activity of PACC for bovine serum albumin (BSA), which has peptide bonds, is good. The degraded rate of BSA is 4.07% by using PACC in stirring reactor. The content of amino acid in BSA after reaction is 2.3 times more than that of amino acid in BSA before reaction when PACC add to glass chromatography column. The content of amino acid in tea extract is 1.87 times more than that of amino acid in tea fumet when tea passes through PACC column.It is important to prepare particles of agarose- Ce (IV ) complexes ( PACC) in this paper. And theoretic significance and actual value is great. The results show that PACC can hydrolyze phosphoester and peptide bonds. Therefore, a new investigative aspect of mimic of phosphoester enzyme and mimic of protein enzyme is obtained. A new method of degrading organophosphorous pesticides in products from farm produce will be found if PACC can hydrolyze organophosphorous pesticides in fruit and vegetable juice. At the same time, a new kind of technology of hydrolyzing protein in beverage industry is gained.