Research Of Microfluidic Chip Fabrication And Application In Food Safty Detection

This thesis includes a review and a research section. In the review, the history ,principle and type of chemluminescens immunoassay(CLIA) is introduced. The progress of analysis method based on microfluidic chip and chemiluminescense immunoassay in recent years is presented. Moreover, the applications of microfluidic chip and CLIA in many fields, such as food safty, environmental analysis, are reviewed. This method has become an increasingly promising analysis method owing to its non-radiation, simple instrumentation, high sensitivity, wide dynamic range, reproducibility, simplicity and rapidity analysis, its automation from computer control.We found that the main condition affect the depth breadth ratio and exactitude is the tempreture of preroast time. So we established a means to frabricate PDMS microfluidic chip in laboratory. The SU-8 photosensitive glue is preroasted at 65 ℃ for 6 hours and 95 ℃ for 2 hours, then it is roasted at 65 ℃ for 5 hours and 95 ℃ for 2 hours after exposed for 12-14 hours. PDMS mixed with condensate in 10:1, then the mixture solidify on the SU-8 mould surface at 65 ℃ for 40 minutes.Luminol can be oxidized by benzoyl peroxide(BPO) and generate a CL signal in alkaline medium in microfluidic chip. So a novel method for BPO detection is founded. The proposed detector for the detection of benzoyl peroxide offers the advantages of sensitivity, simplicity, automation and rapidity. It is also available for on line analysis. The proposed method has been applied satisfactorily to the determination of benzoyl peroxide in wheat flour.The system shows that the benzoyl peroxide is detected linearly in the concentration range from 1×10~-7~1×10~-5 g/mL.The relative standard deviation (n = 8) was 2.8% for 5.0×10~-6g/mL benzoyl peroxide.A novel system for the determination of clenbuterol(CLB) in urine with chemiluminescent enzyme immunoassay and microfluidic chip based on magnetic particles and the indirect competitive ELISA. The linear range of this method is 1×10~-11g/ml--1×10~10g/ml. the recovery of clenbuterol is 85%--105%, RSD is 3.2%(n=11)and sensitivityis 1×10~-11g/ml. The progress ofanalysis can be finished in 15 minutes. As all the program such as enrichment or sample, reaction, washing, separation, detection were disposed on the magnetic particles, this method doesn't need special instruments and technical personnel, the analysis require a little volume reagent and time of detection be cut down also.