| In this dissertation, the ginsenoside Rb3 and ginsenoside Rc, two kinds of triterpeneglycoside constituent, was isolated from the notoginseng folium saponins with adetermined optimal technology condition. And the content of gained ginsenoside Rb3or ginsenoside Rc was high enough to be the standard sample. The structure of theproductions was identified by UV, IR, MS and 13C-NMR.Making the notoginseng folium as the material and ginsenoside Rb3 as the product inpreparative liquid chromatography, the effects of sample quality, the contents and theflow rate of the mobile phase in the separation process were investigated respectively,and thus the suitable chromatography conditions were determined. The content ofginsenoside Rb3 in the product was 97.2%,6mg ginsenoside Rb3 can be obtained from350mg material.The chromatography conditions were aptly adjusted and can be use toproduce ginsenosied Rc the content of which in product was 92% or 97.6%. 5mgginsenoside Rc the content of which was 92% or about 1.5mg ginsenoside Rc thecontent of which was 97.6% can be obtained from 350mg material.Melting point instrument, elemental analytical meter, UV, IR, MS and 13C-NMR wereused to respectively determined the gained ginsenoside Rb3's melting point, contentsof C and H, λmax, several important group compounds, some characteristic structuralcharacters, and all the chemical shifts of C in the molecule of Rb3. The gainedginsenoside Rc was analyzed in the same way. The result of these analysis show theproducts were respectively ginsenoside Rb3 and ginsenoside Rc.This research is significant to the investigation of some new medicine.
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