Study On The Analytical Methods Of Allergenic Disperse Dyes And Sudan Dyes By HPLC And LC-MS

With the improvement of people's awareness on environment and health, the green products are getting more and more attention. Some disperse dyes on textiles and Sudan dyes in foods put a potential threat on human's health. Many countries have made strict rules for the use of these dyes, which become the barrier of entering the market. So the foundation of the precise, simple and quick analytical methods of these dyes has great practical meaning.The article studies the analytical methods of allergenic disperse dyes including disperse blue 1, 35, 106, 124;disperse yellow 3, disperse orange 3, 37;disperse red 1, and Sudan dyes including Sudan I, II, III, IV by HPLC and LC-MS. The chromatographic conditions including the analysis column, composition of mobile phase, detection wavelengths etc. are optimized.By now, there are no standard detective methods for allergenic disperse dyes, the article finds a HPLC method according to Germany draft 522000. These dyes are successfully separated on a C₈ with gradient elution, using the solution of 1% acetic acid and 0.3% THE -acetonitrile as mobile phase, while the wavelength is 280 nm. The RSD of this method is 1.8³.1 %, while the average recovery is 77.5-96.6 % and the liner range is 1¹0 mg/L.For the Sudan dyes, the preparation of the samples in GB/T 19681-2005 has low recovery and reproducibility. Using the commolercial N - SPE, the recovery advanced by 5 19% compared to that using the N-SPE made by ourselves according to GB. The dyes are separated on a C₈ with gradient elution, using the solution of 1% acetic acid - acetonitrile of 1% acetic acid and 20% acetone as mobile phase, while the wavelength is 478 nm. The RSD is 1.1².3% while the average recovery is 90.2⁹6.5% and liner range is 0.1²0.0 mg/L.For Germany draft and GB, the fake positive results occur inevitably. The HPLC methods for the former dyes are all detemined by LC-ESK + )/ MS. The configuration of some dyes is amended by LC-MS and IR. Finally, the samples of 5 textiles and 10 foods are analysed. Among them, 2 textiles are firstly tested as positive sample, finally as fake positive sample by LC - MS. In the 10 kinds of foods, 4 of them are positive, 6 are negative. So for positive samples determined by HPLC, it is necessary to be affirmed by LC-MS.