A Study On The Applications Of Resonance Rayleigh Scattering Technique In Environmental And Pharmaceuticals Analysis

Resonance Rayleigh scattering (RRS) as a new analytical technology has attracted more and more attention for its high sensitivity, simplicity and good selectivity. At present, RRS methods has beenapplied to the study and the determination of biological macromolecules such as nucleic acids, proteins and heparin ect. Furthermore, Resonance Rayleigh scattering has been more andmore used for the determination of trace metal ions in Environmental and some pharmaceuticals.In this dissertation, RRS method has been applied to the analysis of poisonous trace metal ions, such as Hg(â…¡) , Pb(â…¡), and four bile salts pharmacopoeia, such as sodium tauroglycocholate(NaTGC), taurocholic acid Na (NaTC), deoxycholic acid sodium salt (NaDC), cow sodium cholate(NaC). Besides, the spectral characteristics, optimum reaction conditions, influencing factors and and the properties of analytical chemistry have been studied. Furthermore, the reaction mechanism and the reasons of the RRS enhancement have been studied.The research contents are as follows: 1 Mercury(â…¡) -Iodide-Crystal Violet SystemThe spectra of resonance Rayleigh scattering of mercury(â…¡)-iodide-crystal violet system have been studied. The results show that, in 0.02 mol/L H₂SO₄ medium, Hg(â…¡) reacts with I- to form an anionic complexes [HgI₄]^2-, which further reacts with crystal violet to form an blue ion-association complex [CV]₂[HgI₄] through electrostatic attraction and hydrophobic effect, this results in a significant enhancement of the resonance Rayleigh scattering(RRS), the maximum RRS peaks are at 345 nm and 510 nm. The RRS intensity is proportional to the concentration of mercury(â…¡) in the range of 0-4.00 μg/10mL. The method has high sensitivity, and the detect limit...