Elementary Safety Analyasis Of Collagen Protein From Chrome Shavings And Hide Offal

This article aimed to appraise the edible security of collagen protein/peptide which extracted from chrome shavings, through security toxicology experiment. And compare its physical & chemical trait with hydrolyzed collagen and edible gelatine.This study was mainly conducted in three aspects. Firstly, in order to appraise its edible security, the content of moisture, ash, protein, amino acid and chromium (including chromium(VI)) of the collagen protein/peptide(which extracted from chrome shavings) and general bacterial population were determined, which were compared with QB 2732-2005 (hydrolisis collagen protein dried) and GB 6783-94 (skin edible gelatin). Secondly, acute virulence experiment: through the pre-experiment, 72 Kunming-mouses that with half female and male were divided into 12 groups, which were separately filled with different dosage of the collagen protein/peptide and the chromium solution, in order to obtain median lethal doses (LD50) of the collagen protein/peptide and the chromium solution.Thirdly, accumulation toxity experiment and subchronic toxity experiment: the 104 Wistar rats that with half female and male were divided into three groups, including the control (filled with 0.9% saline), the collagen protein/peptide group (filled with different dosage of collagen protein/peptide) and the chromium solution group (filled with different dosage of chromium solution). The security of the collagen protein/peptide was evaluated, by inspecting the accumulate situation of chromium in the internal organs, the general index (symptom, body weight, organs coefficient, food use factor) and generally analyzed index (blood routine, blood biochemistry target) of each experimental group, and the pathology tissue slices were also examined.Result: 1 .The result of physical and chemical index measurement indicated that the moisture and ash content of collagen protein/peptide were increased, the content of protein and the kind of amino acid was reduced. Total chromium content of collagen protein/peptide was 25 times as much as that of the standard. And chromium (VI) content was more than 100 times of that of the standard. 2.The result of acute virulence experiment showed that LD50>15g/kg weight of mouse, the toxicity of the collagen protein/peptide was relatively low. 3.The results of the collagen protein/peptide treated group which treated with medium and high dosages of collagen protein/peptide and chromium treated group indicated that both collagen protein/peptide and chromium contributed to the chromium accumulation of rat's lung and kidney significantly (P<0.05 ), in comparison with control group. In the inferior chronic toxic experiment, the variance tendency of every index of collagen protein/peptide treated group are similar to chromium solution treated group, just a little...