Study On The Interactions Of Azo Dye With Cyclodextrin, DNA And Protein And Analyical Application

CHAPTER 1 The fundamental contents of dye, supramolecule chemistry, cyclodextrin, DNA and protein were introduced. The research advances of the interaction of dyes with cyclodextrin, DNA and protein in recent years were summarized in details.CHAPTER 2 Electrochemical behavior of Reactive Brilliant Red K-2G was studied and a polarographic method for the determination of its have been established. A stable and sensitive linear-sweep voltammetric peak is obtained at -0.78V(vs.SCE) in NaCl (pH6.9) solution. The peak current is proportional to concentration over the range of 7.0× 10^-81.0×10^-4mol/L and detection limit is 6.0×10^-8mol/L.This method is suitable to apply for Reactive Brilliant Red K-2G of the quantitative analysis. The characteristic of the reduction peak current has been studied. At last the mechanism of electrode reaction was preliminary discussion.CHAPTER 3 Polarographic and voltammetric behaviors of Reactive Brilliant Red K-2G were studied. The result shows that Reactive Brilliant Red K-2G has a stable and sensitive reduction peak at potential of about -0.78V (vs.SCE) in the NaCl solution. Interaction of Reactive Brilliant Red K-2G with cyclodextrins was also studied by linear sweep second order derivative polarography. The corresponding inclusion constants were determined by "current method". Inclusion ability between the dye and different kinds of cyclodextrins was compared. A possible inclusion position was preliminary discussed and its theoretical analysis was given.