Studies On Preparing Hemin From Porcine Blood

Heme , the prosthetic group of the hemoglobin, has been used widely and importantly. Oxiding converts heme to hemin when separating heme and globin, so extracting heme from porcine blood is generally preparing hemin. There are two aim for preparing hemin from porcine blood, firstly, it can develop full utilization of porcine blood, secondly, it can provide lots of hemin. On the base of analysis the dominance of methods for preparing hemin on trial, a new method was created, that is the whole technological chain of preparing hemin was separated into three steps: comminutingRBC, separating heme and globin from Hb, extracting hemin, the optimum technology and correlated theories in every step were searched. There were conclusions as follows.A: The best method for comminuting RBC is comminuted RBC by using reagent, the optimum conditions for comminutingRBC were reaction containing chloro-rm and blood corpuscle at a 0. 3:1(V:V)ratio, ethanol and blood corpuscle at a 0.25:1(V:V)ratio, and distilled water and blood corpuscle at a 1.5:1 (V:V)ratio for 30 minute stirring.B: Citric acid is as a separating agent , the optimum conditions for separating techniques were reaction solution containing acetone and blood corpuscle at a 6:1(V:V)ratio, 1M sodium chloride and blood corpuscle at a 7 millilitres :20 millilitres (V:V)ratio at pH 2.80 for 8 hours.C: CMC-Na is as a extracting agent of heme , the optimum conditions for the extracting techniques of heme were reaction solution containing CMC-Na and blood corpuscle at a 1.5g:100 millilitres ratio at pH 6.00 for 8 hours . Results show that the content of hemin was about 5.16%, total output of pure hemin was 95. 1mg/100ml Porcine blood, yield of hemin was 31.3%.