Studies On Screening And Breeding Bacteriocin-like Substance Producing Strain And Its Fermentation Substance

A novel bacteriocin-like substance producing strain was screened from yellow-liquor samples by anaerobic isolation and scrip method and cylinder-dish method and was named CF10. The supernatant of CF10 culture have strong bacteriostasis activity against the gram-positive pathogenic bacteria and the gram-negative pathogenic bacteria and some fungi and microzyme. The strain was identified as Lactobacillus buchneri by morphologic characteristics and physiological-biochemical testing and molecular-biological testing. Excluded the interference of organic acids and hydrogen peroxide, strong bacteriostasis activity was also observed, we belong it to bacteriocin-like substance.In the process of mutation breeding bacteriocin-like substance high-producing strains, CF10 was treated with multiple times compound mutation by ultraviolet (UV) radiation and diethyl sulphate (DES), the relative potency of supernatant had increased 258% and it have stable inheritance.