Preliminary Study On Toxic Effects Of Polycyclic Aromatic Hydrocarbons On Adult, Embryo And Larva Of Sebastiscus Marmoratus

In experimental condition, Sebastiscus marmoratus were exposed to diesel oil soluble fraction (DWSF) at concentrations of 0.02, 0.1, 0.5 mg/L. Samples were taken after exposure for 0, 7, 28, 84 days and transferred to recovery tank for 7, 28 days, respectively. Hepatic EROD, GST activities, content of GSH and biliary PAHs metabolites were detected to investigate possible influence on hepatic detoxifcation system. Effects on reproductive potential of the fish were studied by detecting sex hormone levels in serum and gonad, and vitellogenin (VTG) content in serum.Fertilized eggs were exposed to pyrene (Py), phenanthrene (Ph) and benzo(a)pyrene (BaP) at concentrations of 0.01, 0.1, 1 and 10μg/L,to study effects of polycyclic aromatic hydrocarbons (PAHs) on embryos and larvae of S. marmoratus. Early life stage test indices included mortality, malformation rate, heart beat rate, hatching time, important enzymes activities, and so on. The results showed:1. DWSF exposure influenced hepatic detoxifying enzymes activities in S. marmoratus. Contents of biliary PAHs metabolites were elevated, and the activities of EROD and GST were both induced by DWSF. The enzymes activities may be altered by the defense mechanism of the fish. EROD activities increased firstly and decreased subsequently, which suggested a compensatory or adaptive mechanism occurrence in chronically exposed to the populations.2.There was little change in gonad morphology and gonadosomatic index (GSI) after exposed to the concentrations of DWSF for 84 days. It was observed that 17β– estradiol content of spermary and VTG content of serum were increased in the male fish. Increase of 17β–estradiol content in serum should be responsible for the increase of VTG content. Sex hormone level was declined at the same time in the female fish, which will do harm to the development and mature of ovum. All indices suggested that DWSF exposure had brought reproductive toxic effect, especially caused estrogenic effect to male S. marmoratus.3. PAHs caused toxic effect on early life stages of S. marmoratus. Embryos were exposed to different concentrations of Ph, Py and BaP through water. Mortality rate of...