| Nattokinase, a kind of serine enzyme produced by Bacillus subtilis natto during fermentation, has fibrinolytic activity. Studies showed that nattokinase could obviously dissolve fibrin and blood clot, enhance fibrinolytic system's function of animal and human thrombus model. Nattokinase from fermented food with high efficiency of fibrinolytic was stable in enteron and easy to absorb by intestine. Therefore development of nattokinase for healthy food and oral thrombolysis medicament had a bright prospect.Intra-hollow Ca-alginate microcapsule comes of the Ca-alginate bead. It not only owns the advantages of broad source, inexpensive cost, gentle immobilization condition, easy operation, good biocompatibility, and less resistance to the substrates and the oxygen, but also can overcome the disadvantages of the difficulty that the substrates and oxygen can hardly transfer into the bead core and the limited space that the cells can only grow on the bead surface and in the gel holes. Therefore, the intra-hollow Ca-alginate capsule is a promising immobilization carrier.Microporous starch can absorb materials in its micropore. Put microporous starch into intra-hollow Ca-alginate capsule can augment the content ability of microcapsule.Biomicrocapsule and microporous starch used to immobilize Bacillus subtilis natto and Nattokinase in the same time was studied for the first time in this thesis. Response Surface Methodology was used to optimize the fermentation condition of immobilized Nattokinase. Also the enzyme activity of Nattokinase in fermentation tank and three batches continuous culture fermentation was studied in this thesis. The main results were as follows:1. The preparation of Biomicrocapsule and microporous starch immobilizing Bacillus subtilis natto was studied and the result was: when the concentration Na -alginate 0.8%, CaCl2 1.5%, Microporous starch 4.0%, Xanthan 0.25%, the preparation was feasible and...
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