The Interaction Of Mixed Bacteria With Cellulose Substrate

As advanced renewable energy, hydrogen is attracted more and more attention. In most methods of producing hydrogen, using microbe to ferment biomass such as organic waste water,agriculture solid wastes and organic rubbish in anaerobic condition to produce hydrogen is becoming a noticeable problem all over the world, because of the advantage of getting rid of wastes,protecting environment and producing energy. And using low-cost agriculture organic wastes to produce hydrogen is a hot problem.While the correlative researchs are very few and stay in the stage of library, because of the problem of low utility efficiency and the gain and culture of hydrogen producing bacterium. Basing on the standpoint of interaction in biohydrogen production, the research screened out strains that could use cellulose to produce hydrogen effectively, and mixed them with high hydrogen producing bacterium, hoping to achieve highly hydrogen producing capability, and discuss the key factors that influence the interaction between the mixed bacterium.This research isolated three strains(X9, B2 and C3) that can degrade cellulose to procuce hydrogen, through the test of 16S rDNA, the most similar bacterium with X9 is Clostridium acetobutylicum ATCC 824, and the comparability is about 96%.The YH2 of bacteria X9,B2 and C3 with glucose substrate are 1380 ml/L,1300 ml/L和94 ml/L. X9 can use cellulose to produce hydrogen under the condition of T35-42℃,pH4.0-8.0, and the best result was achieved with T40℃and pH5.0 : total gas production 100ml in 18h, hydrogen producing capability YH2 800ml/L and cellulose degrading ratio 68.9 % with microcrystalline cellulose substrate and 84ml,692.2ml/L and 56.3 % with carboxy methyl cellulose.Under the condition of mixed culture with X9, B2, C3 and B49, we found the hydrogen producing capability in mixed culture is much higher than that in pure culture.Especially the mixed manner of X9 and B49, the best result achieved is total gas production 127ml in 36h, hydrogen producing capability YH2 1810.3ml/L with microcrystalline cellulose substrate and 130ml, 1717.8ml/L with...