A Study On The Preparation And Quality Control Of Radix Puerariae And Other Three TCM Refined Granules

The herbs in cut, such as Radix puerariae, Radix paeoniae, Radix glycyrrhizae, were extracted with water because their main bio-active substances were found to have good solubility in water. The optimum process was determined by a L9(34)orthogonal test with extraction rate of the main bio-active substances and paste-forming rate as determination markers. The optimum parameters were obtained as the results. There are not only water-solvable substances but also volatile oil in flos chrysanthemi. So, all the three determination makers such as extraction rate of chlorogenic acid, paste-forming rate and oil extraction rate were taken into consideration. The optimal water-extraction processes were as follows: for Radix puerariae, the water amount was 10 times of the herb in cut, decocted 3 times and each time was sustained for 1.5 hours; for Radix paeoniae, the water amount was 12 times of the herb in cut, decocted 3 times and each time was sustained for 1 hour; for Radix glycyrrhizae, the water amount was 10 times of the herb in cut, decocted 3 times and each time was sustained for 1 hour; for flos chrysanthemi, all the three factors such as extraction rate of chlorogenic acid, paste-forming rate and oil extraction rate were taken into consideration, and the optimum was: the water amount was 12 times of the herb in cut, decocted 3 times and each time was sustained for 1.5 hours. Both water solution and the volatile oil were collected simultaneously.In the experiment of quality standards, all the four refined granules were determined with the active composition contents by HPLC. The limited contents of puerarin(≥24mg/g), paeoniflorin(≥16mg/g), glycyrrhizic acid(≥30mg/g) and chlorogenic acid(≥17mg/g) were fixed by analyzing the different samples separately. In addition, TLC method was used to identify the four refined granules and the UV spectra method was used to identify the volatile oil in flos chrysanthemi. Moreover, such check-procedures as the moisture, granularity and water-solvability were carried out.