Immunoresonance Scattering Spectral Analysis Of Fibrinogen And Apolipoprotein

PartⅠIntroduction Summarized the application of resonance scattering technology in analytical chemistry and in studying liquid-phase gold. The preparation and identification of gold nanoparticles and its application in biochemical analysis in resent years were introduced. The analytical progress of fibrinogen and apolipoprotein were also introduced. Two hundred and sixty-six references were quoted.PartⅡImmunoresonance Scattering Spectral Analysis of Fibrinogen In pH 6.2 Na2HPO4-NaH2PO4 buffer solution, fibrinogen would combine with its antibody and produce immunocomplex particles that resulted in resonance scattering (RS) effect at 340 nm and 470 nm. The intensities△IRS at 340 nm and 470 nm are proportional to the fibrinogen concentrations from 0.13μg·mL-1 to 5.33μg·mL-1. The detection limits is 0.028μg·mL-1. The method was successfully applied to determination for fibrinogen in human plasm samples, with satisfactory results.PartⅢImmunoresonance Scattering Spectral Analysis of Trace Fibrinogen with Labeling Gold-nanoparticle 9.0 nm gold nanoparticles were prepared by the trisodium citrate and used to label goat anti-human fibrinogen. In pH 6.2 Na2HPO4-NaH2PO4 buffer solution, the immune reaction between gold-labeled goat anti-huamn fibrinogen and fibrinogen took place. The coated gold nanoparticles were released from the goat anti-human fibrinoge and aggregated in the presence of Polyethylene glyco (PEG), which leaded the resonance scattering intensity at 580 nm to enhance greatly. The intensity△IRS is proportional to the fibrinogen concentration from 0.027μg·mL-1 to 1.07μg·mL-1. The detection limit is 1.14 ng·mL-1. The method was applied to quantitative determination for fibrinogen in human plasma, with satisfactory results.