| In this paper, extraction Pomegranate seed oil was studied with supercritical carbon dioxide fluid extraction (SCFE-CO2) technology. We discussed the effects on the extraction rate of the Pomegranate seed oil under different extraction conditions, such as pretreatment of raw materials, the extraction pressure, temperature, CO2 flux, the extraction time, separation pressure and temperature, to find the optimum parameters of Pomegranate seed oil extracting. Meanwhile, the physics and chemistry, fatty acid composition, anti-oxidation, filming and preservation of Pomegranate seed oil by SCFE-CO2 had been analyzed, to provide a theoretical basis for comprehensive utilization of Pomegranate seeds. It includes four parts: the first part, research on the extraction technology of Pomegranate seed oil by supercritical carbon dioxide, including the research on single factor and optimization; the second part, research on the quality and characteristic of Pomegranate seed oil; the third part, discuss the factors affecting the SCFE-CO2, the best process conditions, the oxidation stability of Pomegranate seed oil, the mechanism for processing Pomegranate seed oil by SCFE-CO2, the problems of supercritical fluid extraction and the application prospects of Pomegranate oil; the last part, conclusion.The main experiment results as follows:(1) The pretreatment conditions of Pomegranate seed are important factors that influence SCFE-CO2 technology. Under single factor experiment, the best pretreatment conditions are confirmed. As follows: the crush degree is 40 mu, amount of influx is 300g.(2) Extraction pressure, temperature, CO2 flux, time, separation pressure and temperature are the main factors that influence the SCFE-CO2 technology. Under single factor experiment, the best parameters and scopes are ensured. As follows: extraction pressure 30Mpa, extraction temperature 40-45℃, CO2 flux 15L/h, time100 min, separation pressure 8Mpa, temperature 35℃.(3) The optimum parameters screened out through single factor experiment is regarded as 0 level, then by using quadratic cross rotary design method, the regression equations is established in which X is equivalent to coded values, Y is equivalent to the index of extraction rate. According to the results and statistics analyses, through further optimization and test, the optimum parameters are found out, that is extraction pressure 27Mpa, extraction temperature 30℃, CO2 flux 15L/h, and the maximum of extraction rate is 20.64%.(4) Comparing to the effect of extraction with solvent method, SCFE-CO2 had several virtues. The extraction rate is higher, operating time is shorter. Moreover, the refractive index, acid value, peroxide value and other indicators of Pomegranate seed oil by SCFE-CO2 are superior to the Pomegranate seed oil by solvent extraction.(5) Fatty acid compositions of Pomegranate seed oil were analyzed by GC/MS. There are six components in it and the percentages of them are Palmitic acid (3.21%), Linoleic acid(14.47%), Stearic acid(2.07%), Linolenic acid (1.56%), Punicic acid(63.88%), Eicosapentaenoic acid (8.90%). The content of Punicic acid is the highest,because its nature is very lively, so oxidation reaction will occur easily.(6) The Schaal method is applied to test the anti-oxidation effect of the Pomegranate seed oil against the effect of VE to the lard and salad oil, upon which the analysis on the anti-oxidation effect of seed oil vitro is conducted. The results indicated that the anti-oxidation effect of seed oil is more efficient than VE in vitro, so it could be a preferable antioxidant to dissolve lipid and substitute for VE.(7) The filming experiment of Pomegranate Seed Oil indicated that Pomegranate seed oil is a fine drying oil which may be used in painting industry.(8) Eggs are coated with Pomegranate seed oil to research the effect of preservation. The results indicated that Pomegranate seed oil has obvious effect on inhibit moisture loss inside the eggs; inhibit albumen pH fell and yolk coefficient fell.
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