Studies On Degradation Pathway Of Chlorobenzene In Pandoraea Sp. MCB032

Pandoraea sp. MCB032 can grow with monochlorobenzene as the sole carbonsource. Two gene clusters, which were responsible for metabolizing chlorobenzenes,were found in strain MCB032 through sequence determination and analyses. The oneis the cbs gene cluster encoding chlorobenzene dioxygenase (CbsA) andcis-chlorobenzene dihydrodiol dehydrogenase (CbsB), which transformchlorobenzene to 3-chlorocatechol, the other one is the clc gene cluster encodingchlorocatechol-1,2-dioxygenase (ClcA) and other enzymes, which catalyzechlorocatechol to Krebs cycles intermediate. The key enzymes (CbsA, CbsB, ClcA) inthe chlorobenzenes metabolic pathway have been expressed functionally in this study.CbsA and CbsB transform toluene to 3-methylcatechol, ClcA transforms catechol and3-methylcatechol to muconic acid and 2-methylmuconic acid.