| Chemiluminescence (CL) was defined as electromagnetic (ultraviolet, visible or near-infrared) radiation of the reactant, the production or the intermediate as a result of a chemical reaction. The CL analytical method was established based on the moment radiation intensity or the gross radiation quantities. CL is now considered as a very sensitive analytical method and applied to different fields such as life sciences, geological analysis, pharmacology, clinical medicine, environmental detection and molecular biology etc. because of its high sensitivity, low detection limits, wide linear ranges, simple measure device and apt to realize automation advantage. In recent years, Lv etc. reported that a new chemiluminescence reaction could be initiated when injecting some substances into reaction mixture after the finish of several CL reactions. The phenomenon was named as post-chemiluminescence (PCL) phenomenon and the corresponding chemiluminescence reaction as post chemiluminescence reaction.This thesis consists of a review and a research section.The review includes the chemiluminescence system in which potassium persulfate was used as the oxidant and the application of chemiluminescence systems in the recent year. It was focus on the mechanism and analytical application in luminol- potassium persulfate system.The research gave a presentation of the PCL behaviors of Diltiazem Hydrochloride, Quetiapine Famarate and metal ions in potassium persulfate-luminol system. Chemiluminescence mechanisms were investigated detailedly. Feasibility to use the phenomena to the analysis of these medicines was evaluated and the correlative analytical parameters were also given. The flow-injection PCL analytical method of Diltiazem Hydrochloride, Quetiapine Famarate and metal ions was established.The research section includes three parts.In part one, a new PCL reaction was observed when Diltiazem Hydrochloride was injected into the reaction mixture after the finish of chemiluminescence (CL) reaction of potassium persulfate and luminol. The possible mechanism for the PCL reaction was discussed via the studies of the CL kinetic characteristics, the CL spectra, the UV absorption spectra and the fluorescence spectra of some related substances or reactions. The flow injection PCL method for the determination of Diltiazem Hydrochloride was established. The relative standard deviation (RSD) for the determination of Diltiazem Hydrochloride was 1.6% (n=11, c=6.0×10-5 g/mL). The PCL intensity responded linearly from 3.0×10-6 to 1.0×10-4 g/mL with a correlation coefficient of 0.9966. The detection limit was 3×10-7 g/mL. The method had been applied to the determination of Diltiazem Hydrochloride in the tablets.In part two, a new PCL reaction was observed when Quetiapine Famarate was injected into the reaction mixture after the finish of CL reaction of potassium persulfate and luminol. The possible mechanism for the PCL reaction was discussed via the studies of the CL kinetic characteristics, the CL spectra, the UV absorption spectra and the fluorescence spectra of some related substances or reactions. A new flow injection CL method for the determination of Quetiapine Famarate was established. The relative standard deviation (RSD) for the determination of Quetiapine Famarate was 2.2% (n=11, C=8.0×10-6 g/mL). The linearly responded range was from 1.0×10-7 to 1.0×10-5 g/mL with a correlation coefficient of 0.9957. The detection limit was 3×10-8 g/mL. The method had been applied to the determination of Quetiapine Famarate in tablets.In part three, a new PCL reaction were observed when Mg2+, Ba2+, Cd2+, pb2+ or Ni2+ was injected into the reaction mixture after the finish of CL reaction of potassium persulfate and alkaline luminol. The detection limit were 2×10-5 g/mL, 2×10-5 g/mL, 3×10-7 g/mL, 6×10-7 g/mL, 3×10-9 g/mL for Mg2+, Ba2+, Cd2+, Pb2+ or Ni2+. The PCL reaction was discussed via the studies of the CL kinetic characteristics, the CL spectra and other experiments. The experimental conditions of the CL reactions were optimized and the feasibility using the reaction to analyze these metal ions was evaluated.
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