| Water is the valuable natural resources, and it is the sources of human existence and development. Nitrogen is one of major pollutants of water pollution, and can cause water eutrophication and can directly influence the health of animal and human. Pig farm sewage is the high density organic sewage that is the result of liquid supersession discharged out by live pig and solid excrement and urine in intensify pig farm raised course. With the development of animal husbandry, the sewage of pig farm is a plain main source of nitrogen too. The water pollution is more and more serious, how to deal with and handle the sewages of the animal husbandry feeding effectively, how to make it reach to the discharge standard and recycle and reduce the environmental pollution, those questions are being put in front of us. The denitrification is an important link in the course of sewage disposal. The biological denitrification is the most common method in the sewage denitrification at present.In order to receive the microorganism of the denitrification with stronger ability, 34 strains of microorganism which could heterotrophic nitrify were isolated. Through to the second sift in 34 strains, we found strain B2 could remove nitrogen most rapidly in those 34 strains. In the artificial waste water of the high density, it is 79.94％that ammonia nitrogen is removed in 48h.Strain B2 was identified as Acinetobacter genus.Carrying on the research of relevant properties to B2, we had confirmed the best denitrification condition. The most optimal nitrogen removal condition is 35℃, C/N=19.31, pH=8.10, There is a stronger dependence to the carbon source of small molecule for B2.Inoculating B2 with the sewage of pig farm, nitrogen removal ability of B2 was measured in the real sewage. Because of the real sewage complicated composition, it is 79.94％that ammonia nitrogen is removed. So in real sewage disposal, the course that the bacterial strain should be tamed.
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