Study On The Level Of PAH-Benzo[a]Pyrene In Soil Of The Heavy Chemical District In Tianjin

Polycyclic aromatic hydrocarbons (PAHs), which is a kind of hazardous substance for its' nondegradable, carcinogenicity, accumulation and nonvolatility characteristics, has external effect on the environment. Due to its extensive distribution and long duration, it is hazardous to human's health. Benzo[a]pyrene (BaP) is one of the most representative for the carcinogenicity. The environmentalists have focused on the level of and the form of BaP in the environmental media.In the study, BaP was analyzed. The detectors, the extraction solvents and the extraction methods were evaluated to assess theirs applications for the extraction and analysis of BaP in soil. And the analyzed method of HPLC was selected appropriately.On extraction method selection, the direct solvent extraction method, the Soxhlet extraction method and the Ultrasonic extraction method were campared, and the Ultrasonic extraction method was selected for its quick and simple steps.On clean-up and concentration for the extraction, two methods were used. Firstly, activated silica gel column was used. By HPLC detecting, the shape, the separation and the symmetry of the peak were analyzed to select the weight of activated silica gel and washing solution.But the extraction of soil was purified by the chromatographic column, the peak was not good by HPLC detecting. Then SPE was used. The aspects were analyzed from the preparation of the washing solution, eluting soluting and the mobile phase of HPLC to find out a suitable method.The result dedicated that the column was washed with water/THF (30/70, v/v), BaP was slowly eluted by the THF and the better peak was detected by HPLC. By detected soil, the result showed that the soil was seriously polluted by BaP.