The Study Of Distill Technology Of Immunoglobulin From Bovine Colostrums And Protection Reagent

Immuneglobulin is the most eye-catching colostrums immune factors, mainly used to improve the body's immunity, strengthen the resistance to various diseases. This test of the separation and purification of immunoglobulin, freeze-drying,immunoglobulin under different conditions of stability and the protection of its accession to improve the stability of the effect of the study.The material of this experimentation is bovine colostrums(in 48h),The aim protein is IgG, colostrums is centrifuged 20 min 3500 r/min to degrease, and then sterilize 30 min at 62℃,purified through anion exchange resin column, studied by orthogonal to select vacuum freeze drying parameter: freezing temperature -20℃, freezing time 2h,thickness of sample 8cm, glycin4% and surgar4%, finally found a method to purify the immunoglobulin. The active of IgG is 94%. The rate of distill is 14.6mg/ml.acquire immunoglobulin content above 70.3%.Solution acidity and heated degree have more effect to immunoglobulin activity, This test having studied the stability under 0.5,1.0, 2.0,3.0,4.0in pH and the stability under 60℃, 62℃, 65℃, 70℃, 75℃, 85℃in temperature,The result: With pH value lessening immunoglobulin stability sudden drop,especially extremely unsteady to opposite acid of immunoglobulin. Immunoglobulin is sensitive to fever, Immunoglobulin comparatively stabilize during the period of the low temperature, immunoglobulin only is complete loss activity at 85℃clock water bath five minutes. Through artificial digestion test that affects the immuneglobulin in artificial gastric juice and artificial intestinal fluid, The result is pH value have the largest effect to immunoglobulin activity, followed by digestive effects of time, the least impact is the concentration of IgG and albumen enzyme.Add the protection agent in the solution have protecting effect obviously to the immunoglobulin activity , This test having studied the three kind of protection at the concentration of 5%,10%,15%,respectively,pH value at 2.0,3.0,4.0.studied the activity reserve of immunoglobulin. The result is: with protecting agent thickness's the power-up,protection effect are increasingly obvious,IgG preserve is increasingly high, the protection effect protecting an agent three kinds, glycine> sugar> sorbitol. By the three gene second test that rotating design, three of mixed use protection, the protection of the same amount of the protective effect of immunoglobulin was superior to a single agent, taking into account the synergies between them by a effective role, according to the best adult intake and protection of the protective effect of experimental design, in the pH=2 immunoglobulin extract of the concentration of 2%, IgG immune activity of the protection of the ideal formula: Add glycine of 4%, sorbitol 3.5 percent, sugar 3.5 percent.