Study Of Preparation On High-purify Natural Hot Pepper Erythrophyll By Pre-RP-HPLC

The hot pepper erythrophyll, extracted from Capsicum annuum L., contains many kinds of terpene compounds soluble in grease Capsanthin is allowed and encouraged to be used as one of the medical natural pigments by World Health Organization and some developed countries. T As one kind of edible natural pigment, the hot pepper erythrophyll has the tinting strength strong, safe non-toxic and the very strong nutritional value . Widely applies in the filed of food, medicine, cosmetics and so on.Nowadays, the hot pepper product mostly is thick in the market. The purity of the hot pepper erythrophyll is low , and contains the capsicine, that make the hot pepper erythrophyll contain piquancy, Has limited the hot pepper erythrophyll applications in food and so on . Moreover the hot pepper erythrophyll purity is low, ie mixture,and includes the ingredient uncertainty, that increased the security hidden danger.1 At first,this thesis has studied the separation medium which separated hot pepper erythrophyll .In the research take the silica gel as raw material, responded with the octadecyl three methyl chloride silicon hydride, synthesizes C18 to separate the medium, and carries on the infrared spectrum attribute to the synthesis separation medium. Carries on the filling preparation column with synthesis C18 as the separation medium .2 Has studied the saponified hot pepper erythrophyll thick's temperature, alkaline solution density, the mellow density, tSaponified fluid amount used, saponified time, saponified number of times . Saponified condition: Be treated with 10% methanol sodium hydroxidie solution (the density of methanol is 50%), Add the saponification solution in the ratio of 1g pigment 20mL, at 50℃, 2h each time, 3 times .3 After saponifying the raw materail then carries on the RP-HPLC preparation separation. Inspected preparation factors and so on chromatograph eluent allocated proportion, speed of flow, examination wave length. Preparation chromatograph purification condition : Reversed-phase column(C₁₈,300mm×4.6mm,φ10μm), Chromatograms were monitored at 450nm; the mobile phase was methanol water ethyl acetate(80.75: 4.25:15, v/v); the flow rate was 1mL/min;injection volume 20μL. Finally obtains Capsanthin element andβ-carrot element pure purification has respectively achieved more than 97%.4 Based on the data of prepares the high-purity hot pepper erythrophyll in the laboratory level, Has carried on the technical grade enlargement design variable computation, After obtained the enlargement technical grade design correlation parameter. And has carried on the economical feasibility analysis after the technical grade enlargement design cost. Through the parameter computation and economical feasibility analysis ,Will prepare the high-purity hot pepper erythrophyll for the RP-HPLC industrialization to provide the reference...