Study On The Fermentation Techniques Of The Pyruvate

Pyruvate is an important pathway in the organic acids, widely used products. Using traditional chemical synthesis production, high cost and complex process, cause serious pollution. The fermentation production is low cost, simple process, basic no pollution, and more conducive to industrial production. The acid fermentation process has been optimized study in this article.Author uses Torulopsis glabrata TK418 as fermentation strains, the fermentation determine the best conditions: temperature of 30℃, seeding of 10 percent, speed 220 rpm, a seed flask of 24 h. And determined to iron nitrate as the chemical method of determination of pyruvate. By orthogonal experiment and response surface analysis method to determine the fermentation medium, or glucose as carbon source, the concentration of 106 g / L; ammonium sulfate as the sole source of nitrogen, the concentration of 8 g / L; the carbon and nitrogen ratio was 25:1. By using Box-Behnken centre design, combined with SAS statistical software to determine the optimal concentration of the vitamin combination: thiamine hydrochloride 0.0125 mg / L, nicotinic acid 9.7 mg / L, bio-0.022 mg / L, pyridoxine hydrochloride Alcohol 0.54 mg / L, and confirmed thiamine hydrochloride is the most important factor. Through the medium optimization, the highest output of pyruvate to 68.51 g / L, increasing 22.84 percent. Through adding different layers of KH2PO4 to the medium of fermentation, we find out the optimal concentration of 2 g / L, at least at this time of the by-products.Experiments take different way of comparison in the level of dissolved oxygen in the fermentation tank .the dissolved oxygen determine the best way: 0 ~ 12 h in the control of dissolved oxygen in the level of 30 % ~ 40 %, 12 ~16 h dissolved oxygen in control 90 percent. 16 h to the end of fermentation control in the dissolved oxygen 65 % ~ 75 %. Through the organic nitrogen and inorganic nitrogen and its impact on the fermentation of the dynamics of the orthogonal we further verify the level of nitrogen level as the best source of nitrogen acid fermentation, and with different source of nitrogen under the conditions of acid we get the fermentation Dynamic equation.Identified a laboratory for the extraction of the pyruvate: synergy - complex extraction method. Chelators for TOA, for the coordination of ethyl acetate...