The Application Of The Magnetic Polymer Nanoparticle In Protein Absorption

The development and applications of biological products were seriously hindered by the conventional purification technology for its long-period, low efficiency, and high cost. Magnetic microsphere, as a new kind of purification technology,for its quickness, has been goten more and more attention eficiency and economy. decades. Chitosan magnetic microspheres have attracted considerable atention as they display very important significance and value in theoretical and application aspects. Whilst, owing to the magnetism of the microspheres, separation efficiency is increased in the presence of outer magnetic field, which leads to achieve a cheap and convenient separation in many aspects.It has a broad and wondering application of separation technology based on magnetic carrier in biotechnology and medical research such as cell separation, protein purification, nucleic isolation, targeted medicine, enzyme immobilization, immunological detection.This paper mainly includes two aspects as follows:(1)Prepare the superaramagnetic nanoparticles by coprecipitation. Study on the effect of the pH, temperature and aging time on the morphology and properties of the nanoparticles, and optimize the reaction conditions.The crystal phase and the morphology of the nanoparticles are characterized. And study on the effect of the storage system on the dispersibility of the superaramagnetic nanoparticles.(2)prepare the magnetic chitosan nanoparticles(Fe2O3-CS). The microemulsion includes liquid paraffin as the oil phase, span80 and tween 80 as the emulsifier, and the chitosan-acetic acid solution as the water phase. Glutaraldehyde is the crosslinking agent Study the effect of reaction time, chitosan and crossling agent on the magnetic chitosan nanoparticles. Transmission electron microscopy (TEM), Fourier transform infrared spectrophotometry (FT-IR) and vibrating specimen magnetometer (VSM) DSLwere used to characterize the nanoparticles. The mean diameter of the particles was about 40 nm with favorable dispersibility and good magnetic response.The bovine serum albumin (BSA) was connected onto the surface of the magnetic nanoparticles with the Carbodiimide hydrochloride (EDC) as the activator. The products were then characterized by Atomic Force Microscope (AFM) and ultraviolet spectrometer (UV). The results showed that the nanoparticles had a good adsorbability of the BSA. The isotherm adsorption data of the magnetic chitosan microspheres obeyed the Langmuir model, with a maximum adsorption capacity of 250 mg?g-1 and an adsorption equilibrium constant of 0.007 L?mg-1. The stability of the particles-BSA compound in PBS with different pH was also studied. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) showed that BSA was desorbed from magnetic particles under alkaline condition.