Study On The Enzymatic Hymatic Hydrolysis Of Bagasse To Monosaccharide

Bagasse is a major byproduct from sugar manufacture. Currently, this fibrous raw material has not been fully used but burnt to reclaim its partial solar energy. This practice is neither environmentally benign nor energy efficiency. In order to make full use of this natural resource, this paper has studied the influence of pretreatment conditions on the enzymatic hydrolysis of bagasse. Results of this study will establish a platform for the further generation of ethanol fuel and other chemicals from bagasse.The study began with the composition determination of bagasse following the standard procedure employed by pulping industries. By employing response surface design of single factor analysis, the study then conducted a systematic investigation on the optimal conditions for bagasse pretreatment using peroxide-acetic acid system in term of lignin removal, and the optimal conditions for enzymatic hydrolysis. With the optimal conditions established, the study has further explored the influence of the treatment on enzymatic hydrolysis. Results indicated that the treatment of bagasse using 64.28% H₂O₂-HAc under 74.93℃for 28.46h can reach optimal result. Lignin removal under this optimal condition is 95%. FTIR spectroscopy and X-ray diffraction studies indicated that the pretreatment has led to a decrease in crystalinity of cellulose besides the elimination of lignin. Similarly, the best result of enzymatic hydrolysis of pretreated bagasse can be obtained by using 0.1026 mL cellulase (the density is 12.5mg/mL) in pH 4.4 buffer solution for 36.6h under 50℃. Under the optimal condition, 36.1% organic matter can be dissolved. Ion chromatography data indicated that the enzymolysis fluid contained 18.98% glucose and 6.01% xylose.Results of this study thus showed that bagasse could be a potential natural resource for the production of monosaccharide, which could be further used for the generation of ethanol fuel and other chemical stocks.