Research On Determination Methods Of Ochratoxin A In Wine

Ochratoxin A(OTA)is carcinogenic to rodents and possesses teratogenic, immunotoxic and possibly neurotoxic and genotoxic properties. In 1993, the International Agency for Research on Cance(rIARC) classified OTA as possibly carcinogenic for humans (group 2B). Several surveys confirmed the frequent presence of OTA in grapes products and wine. With the much more wine consumption, the safety of wine is becoming the focus of people's attention, and it is imperative to the research on determination methods of OTA in wine. In foreign countries, there have been standard analytical method for detection of OTA in wine, and legislation for OTA in grapes and grape-derived products. However, there is much less reports about OTA in wine in China. We are lack of standard analytical method for the detection of OTA in wine, and legislation for OTA in grapes and grape-derived products.In this paper, the extraction and determination of OTA from wine had been studied. The purpose of the paper was to develop a simple, accurate and rapid OTA analysis method for measuring OTA in table wine. All of these provided reference for the foundation of analysis standard method of OTA in wine.The main results were as follows:1. Immunoaffinity Column(IAC)was used to extract and purge OTA from wine, the eluents were detected separately by HPLC fluorescence detection and fluorophotometry. Compared with these determination methods. The results showed that HPLC fluorescence detection was suitable for the determination of OTA in wine and grape skin ultrafine-powder, and fluorophotometry was not. Fluorophotometry got fake results. IAC is highly specific for only one target mycotoxin, and simply perfomed, but rather expensive.2. Factors, including various ways of activity, washing solution, elution, on the extracting OTA were valued and simultaneously suitable related parameters were selected and evaluated for precision and accuracy. A basic method of Solid-phase Extraction(SPE)for extracting OTA in wine was established: 10mL wine was firstly brought onto the C18 cartridge which was previously activated by 5mL methanol and 5mL water, later, flushed with 1mL water, after entirely dried, eluted with 5 mL ethyl acetate. The eluent was collected and evaporated till dryness, and the residue was dissovled with 1 mL of mobile phase and determined. The recoveries of this method were between 88.2% and 100.9%, and coefficient of variation varied from 0.93% to 2.19%. This method owns an ideal attribute of simplicity, low cost and rapidly analysis.3. The OTA was detected in demestic wine. The contents of OTA in the samples are much lower than the maximium permitted levels of 2 ng/mL have been estabilished for OTA in wine and grape based drinks in the European Union. Chinese wine is safe.