The Study Of Strengthening Bioremediation Of Petroleum Polluted Sediment

Exploration of oil in the Northwest China led to serious pollution of lands, led to pollution of sediment in the reservoir,and formed potential harm to water resource in this area. The study of strengthening bioremediation of petroleum polluted sediment was made in this paper.Bioremediation was used to deal with petroleum polluted sediment in the experiment.The impact of four methods to strengthen bioremediation was researched in the simulation experiment by adding nitrogen and phosphorus,adding oxygen and mixing round, adding electric field and adding surface-active agent.The main conclusions of the study were as follows.(1)After adding NH₄NO₃,the degradation rate of TPH was 31.15% , higher than that of adding NH₄CI and adding (NH₄)₂SO₄,which showed NH₄NO₃ was the best nitrogen resource in the bioremediation of petroleum polluted sediment.(2) Adding oxygen to the top of water,the degradation rate of TPH in the sediment was 43.52%,only 4% higher than the blank,which showed adding oxygen to the top of water didn't ameliorate bioremediation of TPH in sediment.Adding oxygen to the bottom of the sediment, the degradation rate of TPH improved to 65.20%,which showed that concentration of oxygen was the key factor in the process of bioremediation of petroleum polluted sediment.(3)The degradation rate of TPH in the sediment was 48.97% in the condition of mixing round with adding N₂, the degradation rate of TPH was 46.80% in the condition of mixing round with mechanism, 8%～10% higher than the blank,which showed that mixing round ameliorated the contact of microorganism cell and matter in the circumstance,strengthened the transfer of matter,and improved the degradation rate of TPH in the sediment. (4)After adding electric field,the molecule of TPH moved to the anode,strengthened transfer of matter in the sediment,and improved the activity of microorganism. So the degradation rate of TPH of the group of adding microorganism ang electric field was highest,which was 34.98%, 8% higher than the comparision.(5)After adding biology surface-active agent, the degradation rate of TPH in sediment was 79.01%, 30% higher than the comparision, which showed that surface-active agent improved the removal rates of petroleum hydrocarbon greatly. Tuwen80 was not good at bioremediation of petroleum polluted sediment. It was not suggested to be used.(6)By comparing of the degradation rate of TPH,the measure of adding biology surface-active agent was the best ,the measure of adding oxygen and mixing round was the second ,another three measure was not good and was not suggested to be used. The measure of adding oxygen and mixing round was hard to put into practice in the large-scale bioremediation.If biology surface-active agent could be produced vastly,adding biology surface-active agent to petroleum polluted sediment was a good way to improve bioremediation in sediment.