Effects Of Graphite Carbon Nanoparticles On Cell Growth In Vitro

Objective: To investigate morphologic characteristics of carbon nanoparticles and observe the effect of nanocarbon on cell growth, proliferation and ultramicrostructure.Methods: We tested about the size, shape and potential of CNP with Atomic force microscope, Laser particle sizer and electron microscope. Selected HepG2 cell,L02 cell,Hl7702 cell and 3T3 cell to investigate cell amount with hemacytometry and MTT chromatometry, detect cell cycle with flow cytometry and observe cell ultramicrostructure with transmission electron microscope. We used statistical methods as analysis of variance and Dunnett test in analysis of the result.Results: CNP are spheric particles with the diameter of 20nm. Compared with control group, the three cell group lines expect group HepG2 were significantly improved by CNP in certain concentration and the effect was most when the concentration of CNP was 7.5μ g/ml. Cell apoptosis of 4 cell lines with CNP is significantly lower than the lines without CNP. The transmission electron microscope results of cells with the effect of 7.5μg/ml CNP showed that CNP are well-distributed in cells and there is no damage of cell ultramicrostructure.ConcIusion: CNP have promoting effect to cell promotion and its effect is related to CNP concentration.