| In this paper, seven iV-containing compounds were synthesized and characterized by elemental analysis, IR spectra, 1H NMR and X-ray crystallographic analysis. N-containing compounds coordinated with transitional metal were synthesized and the electrochemical nature and the interaction with DNA were investigated.In the first chapter, the classification of heterocyclic compounds, application and research progress, especially benzoic-nitrogenous heterocyclic compounds, thiazole compounds and the research and application of imidazole compounds were introduced.In the second chapter, with o-diaminobenzene as the original material, five series of compounds, such as amide, benzothiobiazole and benzimidazole derivatives, were synthesized. The compounds were coordinated with metals to form metal complexes. The compounds were characterized by elemental analysis, IR spectra and 1H NMR. Five single crystals of them were obtained and their structures were determined by X-ray crystallography method.In the third chapter, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to investigate the interaction between [Cu(C16H14N2O2)] and salmon sperm DNA. When DNA was added, the peak current of [Cu(C16H14N2O2)] was decreased and the peak potential was not changed. In 0.2 mol·L-1 B-R buffer solution, the binding ratio between [Cu(C16H14N2O2)] and salmon sperm DNA was calculated to be 2:1 and the binding constant was 6.63×105 L2·mol-2.In the fourth chapter, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to investigate the interaction between [Co(bth)2(H2O)4]+ and salmon sperm DNA. An electrochemical DNA biosensor for the detection of short DNA sequence related to the human immunodeficiency virus (HIV) was developed with [Co(bth)2(H2O)4]+ as hybridization indicator. With this approach, a sequence of the HIV could be quantified over the range from 3.2×10-7 mol·L-1 to 2.9×10-6 mol·L-1, with a detection limit of 1.1×10-7 mol·L-1. The electrochemical sensor showed good selectivity.In the fifth chapter, the process of [Cd(C7H6N2)2]2+ on glassy carbon electrode might be a quasi-reversible reaction controlled neither by adsorption nor by diffusion. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to investigate the interaction between [Cd(C7H6N2)2]2+ and salmon sperm DNA. The results show that the mode of the interaction of DNA with [Cd(C7H6N2)2]2+ is intercalative. The novel hybridization indicator, bis(benzimidazole)cadmium(II) dinitrate (Cd(bzim)2(NO3)2), was utilized to develop an electrochemical DNA biosensor for the detection of short DNA sequence related to the hepatitis B virus (HBV). With this approach, a sequence of the HBV could be quantified over the range from 1.49×10-7 mol·L-1 to 1.06×10-6 mol·L-1, with a linear correlation of r = 0.9973 and a detection limit of 8.4×10-8 mol·L-1. The [Cd(C7H6N2)2]2+ signal observed from probe sequence before and after hybridization with four bases mismatch containing sequence was lower than that observed after hybridization with complementary sequence, showing good selectivity. These results demonstrate that the indicator [Cd(C7H6N2)2]2+ provides great promise for rapid and specific measurement of target DNA.In the last chapter, it was summarized for the whole thesis. |
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