Study Of Phthalic Acid Esters In Food And Plastics Rubber By GC-EI/MS And CG-MS/MS

Phthalic acid esters (PAEs ) are widely used as plasticizers all over the world. In the past year, people had thougt that they were less toxic, but recently, more and more investigations have shown that PAEs are Environmental Incretion. Usually, they combine with the food, the air and other things, coming into body through digestion, respiration or skin and leading to the instability of internal secretions. PAEs are toxic and harmful to hunman, so they are regarded as "The second global PCBs Pollutants" now.This dissertation focused on the studies of different analytical methods for PAEs in different samples, such as animal innards, vegetables, plastics rubber and so on. The method was as following: extracted with different solvent against different samples, separated by Florisil diatomite, then analyzed by GC-EI/MS SIM. Furthermore, the analytical methods of PAEs by GC-MS/MS was discussed in this thesis preliminarily.The first chapter, simply introduced the study of PAEs Environmental Incretion in China and abroad, which including the Pollution level of PAEs in different areas and the analytical method of PAEs. Finally, the plan of this dissertation as well as the outlook of the subject was also interpreted.The second chapter, GC-EI/MS was applied to the determine of 14 PAEs simultaneously in animal innards. Firstly, the structures of PAEs fragments and their EI/MS rupture mechanisms were parsed. Then, the different parameters such as the extractant, the eluent and proportion of eluent were optimized during the pretreatment process., at the same time ,the problem of analytical blank was dissussed. Finally, PAEs were analyzed in six animal innards, such as pork liver, duck liver, chicken liver, beef liver and so on, confirming that PAEs have been accumulated in animal innardsThe third chapter, an analytical method was developed for the simultaneous determination of 16 PAEs in vegetables by GC-EI/MS. PAEs were extracted with hexane in an ultrasonic bath and cleaned up on a Florisil column, then were determined by GC-EI/MS with Benzyl benzoate ( BB ) as internal standard. At last, the radish, brinjaul, potato, cabbage and wax gourd were analyzed, providing a effective measure for the control of PAEs pollution in vegetables.The four chapter, developing a GC-EI/MS method for simultaneous determination of 13 PAEs in plastics rubber with external standard method in quantitative. The recoveries, limit of detection, precision and linear range were investigated to pledge the reliability. Furthermore, the quantitative problem of DINP with the isomers was dissussed especiallyThe fiver chapter, the analytical methods of PAEs by GC-MS/MS was discussed preliminarily in this thesis. Take 9 PAEs for example, several parameters for MS/MS such as scan time, parent ion, excitation storage level, CID voltage, the model of resonant and nonresonant and so on were optimized. Finally, the analytical method of 9 PAEs with GC-MS/MS was established. Furthermore, the MS/MS and SIS were compared in Sensitivity and S/N.