Study On The Preparation And Stability Of Lycopene Nanodispersion

lycopene known as an important functional lipid ,has many biological activities such as quenching singlet oxygen, eliminating peroxyl-radicals and regulating tumor cell proliferation. however the poor water solubility of lycopene has limited their using range. Nanotechnology provides a good opportunity to improve the solubility of such functional lipids. Particles in the nanometer range increase effective contact area of gastrointestinal cells, which should lead to improved absorptivity and bioavailability. This paper focuses on the preparation and stability of lycopene nanodispersion, Z-average diameter of lycopene nanoparticles, ranging from 90 to 250 nm by emulsification-evaporation was observed. Its main contents are described as follow:The formula of lycopene nanodispersion was studied through orthogonal experimental methodology. The results showed if Tween-20 was used as emulsifier at a dosage 0.5%(W/W),ethyl acetate was used as organic solvents and the ratio of organic/aqueous phase was controlled at 2:8, the lycopene content in nano-dispersion was 0.7%(W/W),the Z-average diameter of the lycopene in the sample was around 93.87nm.After the observation in 120 days, the remarkable change of the particle size and distribution of lycopene nanodispersion did not arise.It was quite stable.The emulsification-evaporation process of lycopene nanodispersion was also studied. Firstly, We choose the ultrasonic emulsification as nano-prepared method by compared ultrasonic emulsification and high speed distribution on the effect of lycopene nanodispersion. Then optimized the processing technology using absorbance change rate, color and particle size as evaluated index was obtained .The final parameters were as follows: ultrasonic power 468 W, as 72% total power, ultrasonic time 3 s, ultrasonic total time 4.2 min, ultrasonic interval time 2 s, 2 times. The Particle size was 113.56 nm, absorbance change rate was 4.98 %.The Z-average particle size and ultrasonic power showed a good correlation according to particle size obtained by different ultrasonic power. The Z-average particle size decrease with the rise of the ultrasonic power. As different properties may in different nano-sized grade, a desired particle size distribution can be produced in a controlled way by varying the production parameters. It is shows the relationship between Z-average particle size and absorbance change rate. So it is possible using absorbance change rate as evaluated index.The concentration of lycopene nanodispersion assayed by HPLC was 48.7μg/mL, the residual content of Ethyl Acetate of sample was 6.853μg/mL, less than national standard. As for stability of lycopene nanodispersion research, we found it was sensitive to light. The retention rate was far than common lycopene under different light conditions; the higher temperature was, the lower the stability of lycopene nanodispersion; the effect of oxidant on stability of lycopene nanodispersion was big; Vc had little effect on stability of lycopene nanodispersion, conversely has promoting oxidation on certain condition; Metal ions, such as Mg2+,Fe2+,Na+,K+ except Fe3+,Cu2+,Al3+ had little influence on stability of lycopene nanodispersion. The effect of preservative, glucose and citric acid on stability of Lycopene Nanodispersion was very little...