The Effect Of PCP, TPT And Heavy Metal On The Growth And Physiological Parameter Of Plants

It is studied that toxic effect of pentachlorophenol (PCP) and triphenyltin (TPT) on the growth and physiological index of Lemna minor,Spirodela polyrrhiza and Mung bean, the toxic effect of Cu2+ and Cd2+ single and combined pollution on the growth and chrolophyll content of Spirodela polyrrhiza, and the toxic effect of Pb2+ and Cd2+ single and combined pollution on the growth and physiological parameter of Mung bean. The results of experiments were summarized as follow:(1) The result indicates that the 96 h IC50 of PCP to the number of Lemna minor's leaf is 1.18 mg/L, the 96 h IC50 of PCP to the number of Spirodela polyrrhiza's leaf is 1.00 mg/L, the 120 h IC50 of PCP to the number of Mung bean's leaf is 5.74 mg/L, the 120 h IC50 of PCP to the number of Mung bean's root is 7.16 mg/L, the 120 h IC50 of PCP to the length of Mung bean's root is 3.70 mg/L. The result indicates that the 96 h IC50 of TPT to the number of Lemna minor's leaf is 0.10 mg/L, the 96 h IC50 of TPT to the number of Spirodela polyrrhiza's leaf is 0.71 mg/L, the 120 h IC50 of TPT to the number of Mung bean's leaf is 5.84 mg/L, the 120 h IC50 of TPT to the length of Mung bean's root is 3.87 mg/L. The result indicates that the 96 h IC50 of Cd2+ to the number of Spirodela polyrrhiza's leaf is 0.57 mg/L, the 96 h IC50 of Cu2+ to the number of Spirodela polyrrhiza's leaf is 2.39 mg/L. The result indicates that the 120 h IC50 of Pb2+ to the number of Mung bean's leaf is 282.74 mg/L, the 120 h IC50 of Pb2+ to the number of Mung bean's root is 6.47 mg/L, the 120 h IC50 of Cd2+ to the number of Mung bean's leaf is 19.52 mg/L, the 120 h IC50 of Cd2+ to the number of Mung bean's root is 13.86 mg/L.(2) The values of enzymic activity in the plants of PCP are the same with TPT. This showed that, at low concentration, PCP and TPT stimulate the growth of plants, and at high concentration, PCP and TPT inhibit the growth of plants.(3) The influence of the Cu2+ and Cd2+ combined pollution on the growth of Spirodela polyrrhiza is antagonistic effect. At low concentration, the influence of the Cu2+ and Cd2+ combined pollution on the chrolophyll content of Spirodela polyrrhiza is antagonistic effect, at high concentration, the influence is additive effect.(4) The influence of the Pb2+ and Cd2+ combined pollution on the growth of Mung bean's leaf is synergistic effect. At low and high concentration, the influence of the Pb2+ and Cd2+ combined pollution on the growth of Mung bean's root is synergistic effect, at the concentration of 200 mg/L Pb2++20 mg/L Cd2+, the influence of the Pb2+ and Cd2+ combined pollution on the growth of Mung bean's root is antagonistic effect. The influence of the Pb2+ and Cd2+ combined pollution on the chrolophyll content of Mung bean is synergistic effect.(5) The value of polyphenoloxidase (PPO) activity of Mung bean's leaf reduce with Pb2+ and Cd2+ single pollution at low concentrations and increase at high concentrations. The value of PPO activity of Mung bean's root increase with Pb2+ and Cd2+ single pollution at low concentrations and decrease at high concentrations. The value of catalase(CAT) activity of Mung bean's leaf and root increase with Pb2+ and Cd2+ single pollution at low concentrations and decrease at high concentrations. (6) The value of PPO activity of Mung bean's leaf reduce with Pb2+ and Cd2+ combined pollution increasing, at low concentration, the influence of the Pb2+ and Cd2+ combined pollution on the PPO activity of Mung bean's leaf is antagonistic effect, at high concentration, the influence of the Pb2+ and Cd2+ combined pollution on the PPO activity of Mung bean's leaf is synergistic effect. The value of PPO activity of Mung bean's root increase with Pb2+ and Cd2+ combined pollution at low concentrations and decrease at high concentrations, at low concentration, the influence is antagonistic effect, but at high concentration, the influence is additive effect, the PPO activity is 0. The value of CAT activity of Mung bean's leaf and root increase with Pb2+ and Cd2+ combined pollution at low concentrations and decrease at high concentrations. At low concentration, the influence of the Pb2+ and Cd2+ combined pollution on the CAT activity of Mung bean's leaf is antagonistic effect, at high concentration, the influence is synergistic effect, the CAT activity is 0. At low concentration, the influence of the Pb2+ and Cd2+ combined pollution on the CAT activity of Mung bean's root is synergistic effect, at high concentration, the influence is antagonistic effect.