| Capillary electrophoretic enzyme immunoassay with electrochemical detection is an attractive method due to its high sensitivity and low detection limit. The orthoaminophenol(OAP)-H2O2-horseradis hperoxidase(HRP) enzyme-linked immunoassay system was used. The method was used to detect 21-, 39- and 80-mer DNA targets in one single sample.Details of selection for optimum conditions were presented. The system had extremely high sensitivity. HRP can be measured with a detection limit of 1.09×10-12 mol·L-1 (S/N = 3), and a linear range of 2.3×10-123.5×10-10 mol·L-1.This method has been successfully applied to the detection of 21-mer DNA targets. The detection limits (S/N = 3) was 1.2×10-11 mol·L-1, and the linear range is 4.0×10-11-2.0×10-9 mol·L-1. Under the optimum conditions, the 21-mer DNA targets were detected.This method has been successfully applied to the detection of 39-mer DNA targets. The detection limits (S/N = 3) was 2.4×10-11 mol·L-1, and the linear range is 5.0×10-11-1.2×10-9 mol·L-1. Under the optimum conditions, the 39-mer DNA targets were detected.This method has been successfully applied to the detection of 80-mer DNA targets. The detection limits (S/N = 3) was 3.0×10-11 mol·L-1, and the linear range is 5.0×10-11-1.0×10-9 mol·L-1. Under the optimum conditions, the 80-mer DNA targets were detected.This method has been successfully applied to the simultaneous detection of 21-, 39- and 80-mer DNA targets in one single sample. The multiplex assay also provided good specificity without any cross-reaction.A method for indirectly detecting proteins was developed by combination of aptamer ssDNA selective hybridization reaction with corresponding proteins, capillary electrophoresis, enzyme catalyzed reaction and electro analysis, with avidin labeled HRP. The optimum conditions of separation and detection are 2.5×10-2 mol·L-1 pH 8.6 Tris for the buffer solution, 10 kV for the separation voltage, 12 kV and 5 s for the injection voltage and the injection time.A new method of the detection of adenosine was developed by combining capillary electrophoresis with electrochemiluminescence (ECL) based on tris(2,2′-bipyridine) ruthenium(Ⅱ) (Ru(bpy)32+). The optimum conditions of separation and detection are 0.04 mol·L-1 pH 7.40 PBS for the buffer solution, 12 kV for the separation voltage, 15 kV and 5 s for the injection voltage and the injection time, and 1.2 V(vs. Ag/AgCl)for the detection potential. The limit of detection is 1.0×10-8 mol·L-1 (S/N = 3)... |
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