Development Of Enzyme-Linked Immunosorbent Assay For The Determination Of Fenvalerate In Teas

A polyclonal antibody against fenvalerate (FEN) was produced with a new developed hapten by the approach of modifying the characteristic part of fenvalerate. In our method, 2-(4-Chloro-phenyl)-3-methyl-butyric acid was protracted with aminocaproic acid methyl ester. Then, the compound was acidified to produce the hapten. A competitive direct enzyme-linked immunosorbent assay (CD-ELISA) was developed for the rapid detection of fenvalerate in tea. Because of the chiral characteristic of fenvalerate, the standard solution of fenvalerate was treated by 0.5mM NaOH-methanol solution to improve the solubility. In the CD-ELISA, the concentration causing 50% inhibition was 9μg kg^-1 and 15% was 0.5μg kg^-1, and the effects of different chemical conditions (ionic strength, pH value, and organic solvent) were studied. A simple, rapid, and efficient extraction method was developed and 76.67-91.43% recovery of spiked tea was obtained. The detection limits of assay for fenvalerate in tea were less than 1μg kg^-1. The reliability of the CD-ELISA samples was compared with that of GC. The correlation between data obtained using the microwell assay and GC was good (R²=0.9968). Therefore, the developed immunoassay method in this study is suitable for the rapid quantitative determination of fenvalerate in teas.