Se Nanoparticles Mineralization By Prokaryotic Microorganism

This paper adopt the biological mineralized strategy to implement a series of selenium biological mineralized experiments by choosing the microbial system with strongly vitality and fast breeding,meanwhile,exerting sodium selenite as selenium source artificially. It synthesizes red spherical nano-Se by using the escherichia coli, bacillus subtilis, and actinomycete, presents a certain regularity; the experimental results was characterized with the analysis testing method of SEM,EDX, XPS and XRD; discusseing the different microbial system, the possibility, regularity and structural characteristics of the same source of selenium ore a nanometer element Se; establishes the methods of biological mineralization and separation preparation.The experimental results show that Bacillus subtilis and E. coli mineral compound globular nano element Se, with a diameter of 50-100nm; actinobacteria mineral compound globular nano element Se, with a diameter of 100nm-200nm.The effect of single-mass-string Se is better by using the method of Lysozyme combine heating in separating the mineralization. The structure and characteristics of appearance of microbial mineralization nano are related to microbial species, exert the silicon nutrition and mineralization conditions. The experimental results provide experimental basis for further researches on biological mineralized nano element Se and its application.