Theoretic Establishment,Experimental Verification Of The Moving Oxidation-reduction Boundary And Application Research Of Zone Boundary In Electrolyte Solution Boundary

As one of the important branches of electrochemistry, the electrolyte solution boundary has become a very hot topic in analytical chemistry, among them moving chemical reaction boundary (MCRB) and zone boundary have come to be the main development trend in recent 10 years. The main work of this paper is focus on the establishment and verification of the newly moving oxidation-reduction boundary (MORB) and the application study of the capillary electrophoresis. The main points of this thesis are summarized as follows:1,Preliminary establishment of the moving oxidation-reduction boundary.In Chapter 2, based on the concept of MCRB and along with the previous research of other reaction boundary, a model of moving oxidation-reduction boundary was created by the common oxidant and reductant: FeCl3 and KI. Thus the model of MORB was established. From the MORB model, the expression of the velocity of MORB was deducted.2,Experimental verification of MORB.In Chapter 3, to avoid getting the parameters difficult to obtain required in the calculations involved and simplify the computation, a very convenient and efficient data processing method was worked out to validate the model and expression of MORB which submitted in Chapter 2. The experimental results well accord with the prediction, they showed:①The MORB between FeCl3 and KI could be observed clearly between oxidant and reductant by an electric field applied to the large tube.②The ratio of the concentrations of the oxidant and reductant played an important role in the migration of the boundary, and the velocity of the MORB could be controlled by changing the concentration ratio of the oxidant and reductant.③By the expressions of the theory, the influence on the parameters of the velocity were well accord with the predicted ones, which could prove the correctness of the MORB model and expression. Our work was hoped to offer a solid theory foundation and pave a way for further studies.3,Analysis of four ganoderic acids from fermentation mycelia powder of Ganoderma lucidum by capillary zone electrophoresis (CZE)In Chapter 4, a simple and reliable method was developed for the separation and quantitative determination of four ganoderic acids(GAs) from dried triterpene-enriched extracts of Ganoderma lucidum mycelia powder by CZE which was the most commonly used analysis method in zone boundary. Under the optimum conditions, the four GAs reached the baseline separation in 9 min with Glycyrrhetinic acid (GTA) as internal standard. The four GAs and internal standard (GTA) were detected at a wavelength 245 nm. All calibration curves showed good linearity (r2 >0.9958) within test ranges. Limit of detection (LOD) and limit of quantification (LOQ) were less than 0.6 and 1.8μg mL-1, respectively. The relative standard deviation (R.S.D.) values of precision and recoveries was less than 5% and recoveries ranged from 91.4% to 103.6%. It showed that the developed method was effective and stably, it could be regarded as an alternative method for the analysis of the four GAs.