Study Of Polyacrylamide Degrading Bacteria And Degradation Characteristics

Partially hydrolyzed polyacrylamide (Hydrolyzed polyacrylamide, HPAM), is an additive widely used in oilfields. In enhanced oil recovery process, HPAM was used as a polymer flooding process in the oil into the reservoir so that oil and water solution mobility ratio decreased to reduce the viscosity index of water and enhance oil recovery. But the water contained in HPAM will change the physical and chemical properties of water, while HPAM degradation resulting from the monomer acrylamide on humans and animals will be around nervous system can not repair the damage. How to degradation in the polyacrylamide HPAM is very concerned about the flooding problems.Polyacrylamide degradation under natural conditions is spontaneous slow. The bio-degradation is a harmless way to long-term deal with the pollution. In recent years, the mechanism HPAM degradation mainly extracellular hydrolytic enzymes and substances, Some studies showed that the microorganism can produce in hydrolisis HPAM the amido reductases, oxidation and reaction, also can make long-chain HPAM break into short chains,which can be absorbed in the small molecular organic microorganisms, microbial growth for the synthesis of protein and other essential substances. Other studies also showed that some microorganisms can release non-protein class of reducing substances, reducing substances in the non-proteins acting on the polymer, leading to polymer strand breaks induced oxidation.Currently on the mechanism of mixed microbial degradation thought that mixed growth of microbes may constitute a kind of micro-ecological system because all kinds of microbes in the system complement each other common effect. And mixed bacteria in microbial ecosystems synergism was to be studied.The degrading effect of two Hydrolyzed polyacrymide (HPAM) degrading bacteria was studied in polyacrylamide. HPAM degrading bacteria Pseudomonas migula CJ419 and Bacillus subtilis FA 16 was filtered. And Bacterial biomass and HPAM degradation rate were investigated. Focus on testing the growth of two strains collaboration and joint degradation. Degradation of the two strains together when pH, temperature, activation number, inoculation of other parameters were optimized. Preliminary study of two strains degradation mechanism of the polymer And had enlarged test,using Orthogonal experiment Optimizate the test parameters.Polyacrylamide bio-degradation has become the major pollutants. The degradation of polyacrylamide has become a research hotspot in recent years. Polyacrylamide after oil extraction has become the major pollutants produced water, Study and magnifies the treatment of others have some guidance.