Study On Enzymatic Hydrolysis Of Casein

The casein enzymatic hydrolysis modification was studied according to its allergy.The purpose of this study was to hydrolyze casein by trypsin and alcalase, which can reduce the content of casein. At the same time produce some small active peptides, which can reduce the allergenicity of casein and also small active peptides are easy to digest and absorb.Take the molecular weight distribution of small peptides as the index, first by using single factor experiment to ascertain the approximately range of optimum hydrolysis time, pH, optimum hydrolysis temperature and optimum E:S. Then take the molecular weight distribution of small peptides as the index, study the relationship among optimum hydrolysis time, pH, optimum hydrolysis temperature and optimum E:S of enzyme hydrolysis through orthogonality experiment. The optimal condition of trypsin were: 2.5h, pH8.00, 50℃, E:S 14000U/100ml. The optimal condition of alcalase were: 4.5h, pH9.00, 55℃, E:S 15000 U/100ml.The comparison studies showed that hydrolyzate of casein by alcalase obtained degree of hydrolysis, molecular weight 1000~180 were higher than those of trypsin. Under the optimum conditions of alcalase hydroyzate of casein, degree of hydrolysis was 16.99%, molecular weight 1000~180 content was 77.38%. Under the optimum conditions of trypsin hydrozate of casein, degree of hydrolysis was 7.64%, molecular weight 1000~180 content was 59.09%.In order to achieve better results of enzyme hydrolysis, reduce the enzyme hydrolysis of cost and improve the economic practicability, this subject adopted alcalase and trypsin be together as compound enzyme to hydrolysis casein. The optimal condition were: 4.5h, pH9.00, 55℃, the dosage of alcalase was 9100U/100ml and the dosage of trypsin was 3900U/100ml.The comparison studies showed that compound enzyme hydrolysis is more effective than single enzyme hydrolysis. Under the optimum conditions of compound enzyme hydrozate of casein, degree of hydrolysis was 18.81%, molecular weight 1000~180 content was 82.67%.