Studies On The Response Of A Photosynthetic Bacterium To Copper And Its Water Purification

The heavy metal pollution in aquatic system has become a global environment problem, and it is severely influence the physical health of people. It will decrease the influence on the environment if the industrial effluents be disposed by biomaterial before discharge into rivers. Photosynthetic bacterium(PSB) is the most original prokaryotes which widespread exist on the earth and nature, and has a synthesis of the original solar energy system, a general call of bacteria which will not react the photosynthetic oxygen under the anaerobic conditions.It is widely existed naturally in soil, paddy fields, marshes, lakes, rivers and sea. PSB mainly exists in the anoxic areas, the place where the sunshine can transmission into deep of water.The main content is that a stain of PSB was separated and identitied, the effects of treatment with Cu2+ and water quality improved of the PSB were analysised in this article.(一)The water sample was gotten from the upper waters of the Dam of NanWan reservoir in XinYang and the sample sediment from the pound nearby, The liquid enrichment medium was used to culture, and cultivate it isolated from oxygen. And take it pure separate after two times enrichment cultures. Then get three kinds of pure strains in different shapes and colors, give them the different conditions of sunshine oxygen and to test the growth carve of them, then get the conclusion that two of them which grow most quickly in the sunshine anaerobic conditions. The red strain is observed under the microscope, after biochemical confirm it to be a PSB. Next, take the common use PSB consensus primer that designed by which according to 16sRNA consensus sequence. The primer resource is 16rDNA, F5'-TGGTYTGAGAGGATGRYCA*, R5'-CGAATTTCACCTCTACACTCG, PCR Amplification chip estimate about 384bp.The amplification parameters of PCR is 94℃2min,94℃30 s,55℃30 s,72℃30 s,30 cycles,72℃5 min totally have 30 cycles. Take 5μL PCR product to test the electrophoresis on the 1.5% of agarose.PCR Amplification chip estimated as 400bp, and pick the PMMO to sequence. The sequence identity result has reach to 100% on the comparison between Blast alignment and Rhodopseudanonas palustris. The result proved that separated Microorganism is Rhodopseudanonas palustris.(二)The bacterium reached the logarithmic phage, cultured for 48 h, Cu2+ was added into the culture. The growth curve were tested of it, used the continuously pumped fluorescence to test the Fv/Fm, oxygen electrode was used to test the influence of the different effective microorganism Cu2+ to the tested medium respiratory rate. To analyze the microscopic structure change by taking the photos of TEM imagine of SWCNT. Under the effect of high inhibitory concentration, the growth of the PSB was inhibited obviously. Most of the photosynthetic lamellae still can distinguish, some of the inner class cysts of cell expand. The overall envelope structure was damaged seriously even dissolved and made the lamellae within the grand spread inner of the cell. The influence of the C2+ to the antioxidant activities of PSB and to compare with the control group were determined. Every kind of the enzyme active changed obvious.(三)The oxidation pond(not included solid wastes), in a small farm of XinYang countryside, is thrown in a large amount of cultivated Pseudomonas and water lettuce in order to test the effects of water purification. One year later, the experimental results is showed that the TN density of effluent was 120mg/L,COD concentration was reduced by 37.5% at lowest,60.8% at highest. COD concentration compared with raw water, was reduced by 36.8% at lowest,65.1% at highest, and COD concentration in the outflowed water was 670 mg/L at lowest,56.4% at highest. The resultes suggested the chemical characters of water bodies of polluted water were improved obviously by photosynthetic bacteria.