Preparation Of Lycium Ruthenicum Murr Pigments Microcapsules

This dissertation mainly studies on the extraction and antioxidant and microcapsules of pigment from Lycium ruthenicum Murr.Purposing on providing stable foundation for the further research and application of Lycium ruthenicum Murr pigment. The results showed as follows:1. To study the microwave-assisted extraction methods of anthocyanin pigment from Lycium rethenicum Murr.The results showed that the optional conditions for the content of anthocyanin in Lycium ruthenicum Murr including content solvent, microwave power, microwave extraction time, ratio of solid to solution and immersed time duration were studied by single factor test and orthogonal test.The optimal microwave-assisted extraction conditions were as follows:content solvent was (75% HCl ethanol), microwave power was 70 W,extraction time was 20min,the ratio of solid to solution was 1:50, immersed time was 20 h.The optimized extraction technique yielded 15.32% of anthocyanin pigment,and the yield of total anthocyanins was up to 936.27 mg/100 g.The microwave-assisted extraction for anthocyanin pigment in Lycium rethenicum Murr is a time-and cost-saving and highly efficient method.2. The antioxidant activity of pigment from Lycium rethenicum Murr. It has shown strong scavenging ability on O2-, OH, DPPH·.Studied on the effect of Pigment from Lycium ruthenicum Murr prevention and protection to the liver disease caused by the D-galactosamine, CCl4 and AAP others multi-drug. Methods:determined the antioxidant activity of Pigment from Lycium ruthenicum Murr through the self-oxidation simulation method in vitro, used injuried liver by D-galactosamine, CCl4 and AAP and others so as to research the hepatoprotective activity of it.Chemical methods were used to determinate the content of alanine acid transferase and superoxide dismutase in serum and hepatic glycogen of liver, observed the shape of liver cells. The results showed that Pigment from Lycium ruthenicum Murr have certain protective effect on liver injuryed by D-galactosamine, CCl4 and AAP others multi-drug, Pigment from Lycium ruthenicum Murr has high anti-oxidative activity in vivo and in vitro.3. The optimal conditions for the preparation of pigment microcapsules:the alginate concentration was 2.0%, calcium chloride concentration was 2.0%, Embedding of time was 0.5 h, Chitosan concentration was 1.0%,the ratio of wall-material and core-material was 1:3 Under the optimum preparation technology, the embedded rate of Microcapsule was 70 percent.The stability of pigment can be improved significantly after microcapsuled.