Extraction,Purification And Characteristics Of Brown Pigment From Hazelnut Shell

Hazelnut shell is production of waste when hazelnut is been processed, since it will be pollution of the environment and also an enormous waste to the resources. The brown pigment can be get from the shell of hazelnut, whose water-soluble well, and nature is stable, it can be used to drink, soy sauce, pastry food industry and so on as the natural pigment. In this paper, for the raw material of shell hazelnut, studied the extraction conditions of hazelnut shell brown pigment, purification, stability conditions of pigment and external oxidation, and the results of experimental contents are as follows:The technological conditions for ultrasonic-assisted-cellulose extraction of hazelnut shell brown pigment were studied, which included the solvent pH value, solid-liquid ratio, the enzyme concentration, hydrolysis time, ultrasound time and ultrasonic power, etc. By the single factors and orthogonal experimental, the optimum technological parameters of the extraction were as followed: solid-liquid ratio was 1:25, ultrasonic time was 25min, enzyme dosage was 14.0mg/g, ultrasonic power was 600W, on this condition, the pigment absorbance value was 1.032.The adsorption and desorption of the hazelnut shell brown pigment with macro porous resin were studied. The optimum purification conditions were as followed:consistence of adsorption liquid was 2.0mg/mL, pH value was 6.0, flowing rate ether on adsorption was 1.0mL/min, and elution of ethanol was 80%,150mLcolumn volume, flowing rate ether on desorption was 0.5mL/min. The color value of purified pigment was 1.39 times higher than that of the beginning. Purified Hazelnut shell pigment preliminary qualitative experiments showed that it was a kind of flavonoids.The relevant experimental results about the stability of the purified hazelnut shell brown pigment showed that:hazelnut shell brown pigment was sensitive to the out door natural light and easy to decompose; it was hest-stable; it can stable exist under the weakly acidic, neutral and alkaline conditions; most of metal ions on hazelnut shell brown pigment little effect, but Fe2+, Fe3+, Cu2+, Zn2+, Al3+were sensitive to the pigment, which will lead to pigment precipitation occurs, and color changed., the Fe2+, Fe3+ had great impact, which made the pigment absorbance increased by 1.004 and 1.096.Hazelnut shell brown pigment oxidation in vitro experiments showed that the pigment had scavenging effects on hydroxyl radical, superoxide anion radical and aliphatic radicals on lipid-per oxidation. Hazelnut shell brown pigment removal ability of hydroxyl radical was also much better thanVc 22.9%.The results suggested that Hazelnut shell brown pigment can be used as natural anti-oxidants.