Fabrication And Application Of Silver Nanoparticles Chemically Modified Electrode

The research of The chemically modified electrode(CME) stemmed from the late 60s and the early 70s, caused it along with the scientific research worker to the chemical modification electrode's deep research to be independent from other disciplines, developed into an emerging discipline. Until now, The study of chemically modified electrode has become the most active areas in the field of electrochemical domain. The granule size of the precious metal nanometer granule belongs to a nanometer level, therefore which has some special nature. Because raw material price is cheap, and easy to prepare, the silver nanoparticles has aroused researcher's enormous interest on application of the modified electrode. This article chooses the silver nanoparticles to take the dressing agent, used electricity chemistry method to prepare the silver nanoparticles modified electrode, and using in biological member and anticancer treatment medicine research. The present paper main research work includes:1. Fabrication and electrochemical behaviour of silver nanoparticles modified electrode.Uses the embedding method to fix silver nanoparticles (AgNPs) in the glassy carbon's(GC) surface with glutin(Glu). Then a steady glassy carbon electrode modified with silver nanoparticles(Glu/AgNPs/GC)was fabricated. The performance of this modified electrode was studied by optics and electrochemistry method. Meanwhile the electrode's redox peak currents and potential were influenced by different concentration of silver nanoparticles and glutin in this paper. The surface coverage of AgNPs on the modified electrode 1.5862×10-9 mol/cm2 was calculated. Under select conditions, relatively standard deviation of peak currents is 3.15% with parallely detemine eight times in 0.1 mol/L HCl-KCl containing 1×10-4 mol/L [Fe(CN)6]3-/4- solution. The Glu/AgNPs/GC displayed good stability and electrocatalytic activity.2. Direct electrochemistry of hemoglobin immobilized on Glassy carbon electrode modified with silver nanoparticles.In chapter 3, hemoglobin (Hb) was fixed to the surface of a glassy carbon which had modified by the silver nanoparticles (AgNPs) using Sol-Gel technology, a steady Sol-Gel/Hb/AgNPs/GC electrode is obtained. the Sol-Gel/Hb/AgNPs/GC electrode preparation conditions were optimized. The electrochemistry behavior of the Sol-Gel/Hb/AgNPs/GC chemical sensor in B-R (pH=4.1) and the direct electron transfer mechanism of Hb were discussed, finding that silver nanoparticles (AgNPs) kept the biological activity of Hb. Moreover through alkali effect of silver nanoparticles, the direct electron transfer between Hb and the electrode finished. The further result showed that fixes can also maintain in silver nanoparticles modified electrode surface Hb it to the H2O2 biological electrocatalysis activeness. In 3×10-6~2×10-3mol/L the scope, the H2O2 return to original state peak electric current assumes the linear relationship with its density, the lowest examination limits for 6.7×10-7 mol/L (S/N=3). Its research content has the important meaning to the clinical medicine and the life sciences.3. Fabrication and application of polypyrrole-silver nanoparticles modified electrode.In chapter 4, silver nanoparticles was modified on the surface of a glassy carbon (GC) electrode by polypyrrole, a steady PPy/AgNPs/GC electrode is obtained. Its optimum experimental conditions were found. The electrochemical behavior of the PPy/AgNPs/GC chemical sensor in HCl-KCl (pH=1.0) and the direct electron transfer mechanism of silver nanoparticles were discussed. The modified electrode has been applied to the determination of Mitoxantrone. The study is important to life science and clinical medicine.