Preparation Of Quinolones Molecularly Imprinted Polymer Microspheres And Study Of Its Specific Adsorption Performance

Molecularly imprinted is a newly developed technique which is used for preparing molecular discriminating material with selective recognition for the target (template) molecule. In recent years, molecularly imprinted polymer develop at a rapid rate in various fields because of the unique advantages of specific recognition, and high tolerance for acid, alkali, and organic solvent being stable at high temperature and long use life.The rapid development of livestock in our country brings us significant economic benefits and necessities of life, but at the same time, it brings us serious problem of environment. Especially the problem of antibiotic residue in environment resulting from veterinary drugs in feed is impermissibly high is becoming a cause for concern. The sensitive and selective detection technology for antibiotic residue which in environment, feed and food (meat, eggs, dairy products et al) has significances to environmental protection, food safety and human health. In this paper, molecularly imprinting polymer microspheres which can recognize selectively quinolones were prepared by molecular imprinting technique. Specific performances of the polymer microspheres were studied. The polymer microspheres expected widely-used to sample pretreatment for antibiotic residue of quinolones veterinary drugs. The main contents of this paper were as follows:1.This thesis has introduced the molecularly imprinting technique. The application of MIP in environmental is highlighted. The existent problem were discussed and the bright foreground were stated.2.Two new molecularly imprinted polymer microspheres were prepared via precipitation, with methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker in acetonitrile solution using gatifloxacin and levofloxacin as the template molecule and 2, 2′- azobis - isobutyronitribe as the initiator. The polymer microspheres were characterized by infrarew spectrum and scanning electron microscope, pointed out the differences in the structure and pattern of the MIP and NIP. The medium grain size of microspheres is about 100nm. The optimization of reaction condition were studied and the optimal proportion and condition of reagent were obtained. The interaction between template molecule and methacrylic acid was studied by UV spectroscopy and the probably reaction mechanism was conjectured.3.Static adsorption properities of molecularly imprinted polymer microspheres was determined systemically by equilibrium experiment. Adsorption kinetics curve showed that saturated adsorption was achieved in 40 minutes. The results of adsorption isotherms indicated that the maximum absorption has significant difference between imprinted polymer microspheres and blank microspheres. Scatchard analysis showed that two classes of binding sites were formed in the molecularly imprinting polymer under the studied condition. There is only one class of binding sites in the blank polymer microspheres. The selective adsorption experiments study of eight quinolone analogues showed that the imprinted polymer microspheres exhibited high capability for the template and the analogues. The polymer microspheres could be used in cycle.4.The main contents concerned in this dissertation were summarized, the problems need to be resolved father were brought about, and the future work has been suggested.