| With the deterioration of eutrophication, the problem of microcystins produced by some algae has become a highlight research topic all over the word. But routine monitoring of microcystins in natural waters is very difficult because the concentration of the toxin is low and the detection method is usually complicated. It is urgent to develop a rapid, sensitive and efficient microcystins detection technology.At present, detecting methods for microcystins are biological testing, chemical analysis, immunization testing and so on. Overall, all these current detecting methods for microcystins can not meet the practice demands. the high cost of detection, the complex and time-consuming sample preparation, and the bulky equipments. all these limited the number of routine monitorings and its widely use.In this study, A gold immuno-chromatographic assay(GICA)for detection of microcystin-LR in waters was developed with the sample pad, the conjugate pad, NC membrane for purified Goat-anti-mouse IgG and McAb to MC-LR. This paper aims to optimize test system by changing the antibody-colloidal gold conjugates, the test line's position, antibody's concentration on the test line and the marker material and observing the influences of these parameters on the test system.The new dipstick sensor, in form of strips, is capable of detecting limit down to 8 ng/mL for microcystin-LR, and the samples can be detected at the test site within 10 minutes, without having to bring samples back to the laboratory or need any equipments, trained technicians. The method producing GICA for microcystin-LR could be used for reference in the development of the detetion of other environmental toxins.
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