Studies On Reutilization Of Cattimycin Waste Mycelium As Nitrogen Source

Mycelial wastes of antibiotic production is both resource and pollutants, which must be treated reasonably. Mycelial wastes used as subtitute of nitrogen source for antibiotic production can reduce emissinos of mycelial wastes and environmental pollution. It is also can save grain crops and reduce the cost of antibiotic production. This is significant today for which environmental pollution and food shortage is becaming more and more serious, it also meet economic development mode of reduce-resuse-recycle and energy conservation and emission reduction. This paper fouse on the feasibility and effects of waste mycelium of carrimycin as nitrogen source for carrimycin production and effects. Main results are as follows:1.The concentration of dry mycelium was studied. The best concentrations of mycelial wastes as sole nitrogen source and sole organic nitrogen source were 4% and 2%, the titer of carrimycin were 77% and 91% of control respectively. On the basis of elemental analysis, the composition of medium optimized with an orthogonal experimental was strach 6%, myceliual wastes 4%, NH4NO3 0.6%, CaCO3 0.5%, MgSO4 0.1%, NaCl 1%, KH2PO4 0.01%, glucose 0.5%. HPLC analysis showsed that components of carrimycin which produced with mycelial wastes was the same with control.2.Effect of pretreaments on wet mycelium for carrimycin production was studied. The results showed that acid hydrolyzed and autoclaved mycelium were benefit for carrimycin production. The optimal hydrolyzed condition was 4h at 30℃and pH4 with Hcl and carrimycin titer attained 110% of control. The optimal autoclaved condintion was 20 min at 135℃(0.21 MPa), and carrimycin titer attained 88% of control. Compared to control, the percentages of total isovalerylspiramycin and isovalerylspiramycinⅢproduced with acid hydrolyzed mycelium were same, the percentage of total isovalerylspiramycin of carrimycin produced with autoclaved mycelium was decrease by 14%.3. Scale-up experiment carry out in 12L fermentor. Results showed that autoclaved mycelium was benefit for carrimycin production, the concentration of mycelium is not more than 2%, otherwise, strain growth would be severely inhibited. With less concentration of mycelium, carrimycin titer was more. Carrimycin titer attained 1074 U/ml with 1% mycelium and 1.4% fish meal, which was 61% of control and 33.5% higher than that of 2% mycelium.