| The research object is to study on reconstitution of Mortierella Fermentation of GLA.Study its culture medium,culture conditions,ultrasonic extraction, purification of the urine bag study to determine the optimum conditions, provide a reference for industrial production.Mortierella deep fermentation medium and culture conditions GLA optimized to GLA production index, to determine the best culture medium and conditions.In the single factor, based on a SAS8.2 analysis,to determine the best medium: glucose, 7.66%, 0.2% sodium citrate, potassium dihydrogen phosphate 0.2%, 0.05% magnesium sulfate, ammonium sulfate 0.26%, pH5.91 ; in the single factor basis, using analysis of variance to determine the optimal culture conditions: fermentation temperature is 28℃, fermentation time was 7d, liquid volume 50 mL, 8% inoculum;GLA synthesis of its characteristics were studied for the effective increase GLA production. Research shows that by using the temperature sub-culture, can effectively promote the synthesis and improve the GLA GLA production. Sub-culture conditions were: 28℃cultured for 5 days, 20℃culture for 2 days.Ultrasonic assisted extraction GLA, obtained through research on the influence of decreasing order of GLA production in order to liquid ratio>ultrasonic treatment time>ultrasonic power, based on a single factor quadratic response surface analysis of rotation design, may the best extraction process: ultrasonic time of 40min, liquid ratio of 8:1, ultrasonic frequency of 366KHz, the best yield was 899.26mg / L.Urea inclusion of purified GLA. GLA content of the evaluation, the use of orthogonal experiments the optimum conditions for inclusion, using gas chromatography.The best experimental conditions for inclusion were: urea: fatty acids as 4:1 (W/W), fatty acid: ethanol 1:12 (W/W), inclusion temperature of -15℃and inclusion time was 12h, microbial fat content of 47.1% in the GLA.
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