The Preliminary Study In Effect Of Atmospheric Particulate Pollutants On Embryonic Development

Objective: This study was aimed to analysis the Fujian province people date, research meteorological conditions and pollutant sending rate of birth defects, understanding the association in these factors and the incidence of birth defects; further establish the ICR pregnant female mice model of exposure to atmospheric particulate pollutants, and investigate the effect of pollutants on mice embryonic development, then explore the pollutants influence on the carcass concerning the expression of CYP1A2 genes.Methods: Epidemiological Investigation: collected Births data of Fujian Province from 1996 to 2006 ,explore atmospheric pollutants and meteorological factors associated with the incidence of birth defects; Vivo studies: Clean ICR pregnant mice were divided into 4 groups: high dose group (170mg/kg), middle dose group (17mg/kg), low dose group (1.7mg/kg) and PBS control group, i.e. according to prescribed procedures, we used the abdominal cavity exposure route, exposure after end, check the situation of pregnancy and fetal development of the situation, and then collected maternal and fetal liver histopathological examination and liver cell DNA damage detection; Vitro studies: Rats'liver cells exposed to different doses (5,25 and 50μg/ml), whether the pollutants influence CYP1A2 gene expression by RT-PCR.Results: Epidemiological Investigation: by analyzing the population in Fujian province and meteorological data, wind speed, humidity and air pollution index correlated the rate of birth defects, then the API accord with incidence of birth defects; Vivo studies: Pregnant mice exposured to airborne particulate pollutants can cause adverse pregnancy outcomes, compared with the control group, Poisoned group, pregnancy rate and stillbirth rate of absorption increased, live births rate reduced, the difference was statistically significant (P<0.05) ; histopathological examination showed exposed groups of pregnant mice and fetal liver, lung tissue occurred in different degrees of pathological damage (P<0.05); Vitro studies: comet assay results showed that female rats exposed groups showed varying degrees of liver cell DNA damage (P<0.05); MTT measure the rat liver cell proliferation situation, different doses could promote cell proliferation, the difference was statistically significant (P<0.05); RT-PCR results showed that poisoned 48h, 25 and 50μg/ml of liver poisoned gene expression levels than the control group increased, the difference was significant (P<0.05), poisoned 72h, 50μg/ml CYP1A2 gene expression in liver cells exposed groups compared with control group levels increased (P<0.05).Conclusion: The present study suggests that wind speed and API may affect the population birth defects as a risk factor, particulate pollutants can cause adverse pregnancy outcomes, resulting in maternal, fetal liver and lung pathological damage, and maternal DNA to a certain degree of liver cell damage; rats'liver cells exposed to atmospheric particulate pollutants, affected cell increase and CYP1A2 gene expression.